Fig. 4

Detection of secreted endoglucanase enzymes. a CMC-hydrolysis spot assay. Positive control (P): cellulase from Trichoderma reesei ATCC 26921 (5 μg of total proteins; determined by Bradford assay). Negative control (N): 90% (w/v) (NH₄)₂SO₄ solution. Concentrated supernatant (S): B. coagulans cell-free supernatant concentrated by adding 90% (w/v) (NH₄)₂SO₄ (5 μg of total proteins; determined by Bradford assay). b SDS-PAGE of Azo-CMCase positive fractions obtained by cation-exchange (CEC) and size-exclusion chromatography (SEC). Molecular mass markers (M)