Fig. 4

Determination of cohesin-dockerin specificity by affinity-based ELISA. The 96-well ELISA plates were coated with the desired CBM-Coh fusion proteins and variable concentrations of Xyn–Docs were applied to detect specific cohesin–dockerin interactions. Doc dockerin, ScaA1 5 scaffoldin name followed by the number (position) of the cohesin. Type II cohesin interactions are shown in green, Type I in light khaki and group-R scaffoldins in pink. The strength of interaction (color intensity squares) was determined according to the OD results as defined in the “Methods” section (the stronger colors represent strong interaction). The cohesins (left column) and dockerins (upper row) appear in the table according to phylogenetic relationships with bootstrap values represented for cohesins