Fig. 1

The catalytic promiscuity in tyrosinase oxidizes veratryl alcohol to veratraldehyde. Gas chromatographic (GC) profile and mass spectra of the catalytic product using veratryl alcohol as the substrate in the absence (control, blue line) and presence of 100 unit tyrosinase (reactant, red line). The analyte was extracted with ethyl acetate. The chromatographic peaks were identified by the retention time and mass spectra library (NIST02). Authentic standard chemicals were used to double check the identified substrates and products (standards, black line). Inset: (i) mass spectrum and (ii) magnification of the peak at 10.95 min, and (iii) mass spectrum of the peak at 14.63 min