Fig. 4
Decomposition of guaiacyl glycerol-β-guaiacyl ether (GGE) by the catalytic promiscuity of tyrosinase. a HPLC profile of GGE and vanillin in the control without tyrosinase and in the reaction sample which includes tyrosinase. The chromatographic peaks were identified by the retention time and authentic standard chemicals. b GC–MS of the catalytic product when using GGE as the substrate in the presence of 500 unit tyrosinase (red line). Black line represents authentic vanillin standard. Inset: mass spectrum of the peak at 10.31 min c Reaction scheme illustrates that the catalytic promiscuity cleaves the Cα–Cβ bond in GGE