Fig. 8

clr-2 plays a major role in mannan and xyloglucan (XG) degradation. a–c Growth phenotypes of WT, Δgh5-7, Δclr-1, Δclr-2, Δxlr-1, Pc1 (Pccg-1-clr-1), Pc2 (Pccg-1-clr-2), and Px1 (Pccg-1-xlr-1) strains of N. crassa in the medium containing glucomannan as the sole carbon source after growth for 4 days. a Photograph of 3 mL cultures with replicates in 24-well deep-well plates. b Fungal dry weights after 4 days. Bars represent standard deviations. The asterisk indicates a significant difference from WT with an unadjusted p value of <0.003 using one-way ANOVA. c Secreted protein in culture supernatants (SN) relative to WT. Bars represent standard deviations. The concentration of secreted protein is shown relative to WT, which was set to 100%. d Growth phenotypes of WT, Pc1, Pc2, and Px1 strains of N. crassa in the medium containing pure mannan as the sole carbon source after growth for 4 days. e Growth phenotypes of WT, Pc1, Pc2, and Px1 strains of N. crassa in the medium containing XG as the sole carbon source after growth for 4 days