Fig. 3
PgGUX has xylan glucuronosyltransferase activity in vitro. The assay was performed with UDP-GlcA, acetylated xylan without [Me]GlcA decorations, and microsomes from N. benthamiana expressing PgGUX or the control GTL6 protein. Products of the in vitro glucuronosylation were digested with xylanase GH11 and analysed by PACE. The enzyme generates xylose, xylobiose, plus the UX4 oligosaccharide if any GlcA is present on the xylan [12]. Average degree of glucuronisation was 10.9%. *Denote a background bands