Characterization of a heat-tolerant Chlorella sp. GD mutant with enhanced photosynthetic CO2 fixation efficiency and its implication as lactic acid fermentation feedstock

Table 1 Comparison of C and N content between Chlorella sp. GD wild type and mutant M4

Condition	Strain	C%	N%	C/N	Reducing sugar after acid hydrolysis
Condition	Strain	C%	N%	C/N	Glucose (%)	Xylose (%)	Total sugar (%)
Air	WT	43.89 ± 0.38b	6.56 ± 0.11b	7.8 ± 0.09c	16.47 ± 0.98c	8.9 ± 0.51b	25.37 ± 1.03c
Air	M4	48.82 ± 0.24a	7.17 ± 0.04a	7.94 ± 0.05c	31.09 ± 1.65a	8.6 ± 0.84b	39.69 ± 3.62a
6% CO₂	WT	49.24 ± 0.60a	5.06 ± 0.08c	11.63 ± 0.09a	23.17 ± 1.89b	11.16 ± 0.99a	34.33 ± 2.85b
6% CO₂	M4	50.33 ± 0.72a	5.52 ± 0.13c	10.69 ± 0.17b	34.05 ± 2.32a	10.97 ± 0.83a	45.02 ± 2.76a

C and N content (%, w/w, dry weight) and C/N molar ratio of Chlorella sp. GD wild type (WT) and M4 mutant after 4 days of aeration with air or 6% CO₂ using laboratory bottle, and their reducing sugar content after acid hydrolysis. The data are presented as mean ± SD (n = 3) and the different letters following the SD indicate the significant difference at P < 0.05

ISSN: 2731-3654