Fig. 4
From: Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae
Effect of increased aldolase activity on transgenic C. vulgaris cells. a Growth curve of transgenic lines and wild-type (WT) cells under controlled growth conditions (ambient CO2 concentration, continuous light of 40 μmol m−2 s−1). b Time course of chlorophyll a concentration in transformants and WT cells. c Photosynthetic O2 evolution and respiration rates in transformants and WT cells. Both O2 evolution rate and respiration rate were measured in fresh Basal medium supplemented with 5 mM NaHCO3. The true rate of O2 evolution equals the sum of the net rate of O2 evolution (i.e., the observed rate of O2 evolution) and the dark respiration rate. Error bars represent SD (n = 3). Asterisks show significant difference from WT cells (t test, *P < 0.05)