Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity

Table 1 Summary of the cellulosomal cellulases from C. thermocellum analyzed in this study

Enzyme	Locus tag Cthe	Glycoside hydrolase family	Sub-family	Catalytic mechanism^#	References
Cel48S	2089	GH48		Inverting	[24]
Cel9K	0412	GH9		Inverting	[25, 26]
Cbh9A	0413	GH9		Inverting	[27]
Cel8A	0269	GH8		Inverting	[28]
Cel5E*	0797	GH5	4	Retaining	[29]
Cel5-26H	1472	GH5 GH26	25	Retaining/retaining	[30]
Cel9-44J	0624	GH9 GH44		Inverting/retaining	[31]
Cel9N	0043	GH9		Inverting	[10]
Cel9P	0274	GH9		Inverting	[17]
Cel9T	2812	GH9		Inverting	[32]
Lec9A	2761	GH9		Inverting	[17]
Cel124A	0435	GH124		Inverting	[33]
Cel5B	0536	GH5	1	Retaining	[34]
Cel5G	2872	GH5	1	Retaining	[35]
Cel5L	0405	GH5	1	Retaining	[17]
Cel5O	2147	GH5	1	Retaining	[36]
Cel9D	0543	GH9		Inverting	[37]
Cel9F	0543	GH9		Inverting	[38]
Cel9Q	0625	GH9		Inverting	[39]
Cel9R	0578	GH9		Inverting	[40]
Cel9U	2360	GH9		Inverting	[17]
Cel9V	2760	GH9		Inverting	[17]
Cel9W	0745	GH9		Inverting	[17]
Lec9B	0433	GH9		Inverting	[17]

ISSN: 2731-3654