Table 6 Analysis of variance (ANOVA) of β-glucosidase enzyme production by B. subtilis RA10 by using Minitab 16 software based on L25(55) standard orthogonal array experiment
S. no. | Factors | DOF | Sum of squares | Variance | F value | P value | Contribution (%) | Rank |
|---|---|---|---|---|---|---|---|---|
1 | Carbon source | 4 | 477.527 | 119.382 | 16.04 | 0.00 | 52.12 | 1 |
2 | Organic N source | 4 | 65.586 | 16.396 | 2.2 | 0.00 | 7.16 | 4 |
3 | Surfactant | 4 | 201.971 | 50.493 | 6.78 | 0.00 | 22.05 | 2 |
4 | pH | 4 | 36.374 | 9.094 | 1.22 | 0.00 | 3.97 | 5 |
5 | Inorganic N source | 4 | 104.898 | 26.224 | 3.52 | 0.00 | 11.45 | 3 |
Error | 54 | 29.78 | 7.445 | 3.25 | ||||
Total | 74 | 916.135 | 100.00 |