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Table 1 Description of genes targeted in this study

From: Agronomic performance of Populus deltoides trees engineered for biofuel production

Name Genea Description Typeb Sugar Releaseb Yieldb Rametsc
CAD Potri.009G095800 Cinnamyl alcohol dehydrogenase, catalyzes the formation of coniferyl or coumaryl alcohol from their respective aldehydes [58] KD + + 12(C), 12(T)
DUF231 Potri.009G072800 Domain of unknown function, a member of the Trichome Birefringence-Like (TBL) gene family, possibly responsible for O-acetylation of hemicelluloses [49] OE + + 12(C), 12(T)
DUF266 Potri.011G009500 Domain of unknown function, possibly acting as a glycosyltransferase [53] OE + + 12(C), 5(C), 5(T)
EPSPS Potri.002G146400 5-enolpyruvylshikimate-3-phosphate synthase, key enzyme in biosynthesis of aromatic amino acids [59] OE + = 12(C), 12(T)
EXT1;EXT2 Potri.001G020100
Extensin, a basic hydroxyproline-rich glycoprotein localized to the cell wall [60] KD + + 12(C), 12(T);
EXT2: 12(T)
GAUT12 Potri.001G416800 Galacturonosyltransferase targeted to Golgi and involved in xylan and homogalacturonan biosynthesis [61] KD + + 12(C), 12(C), 12(T)
HB3 Potri.011G098300 Transcription factor belonging to the HDZIPIII family, with high expression in xylem tissue [62] KD + + 12(C), 14(T)
IQD10 Potri.001G375700 Calmodulin-binding protein with IQ amino acid-rich region with high expression in tension wood [63] KD + + 11(C), 13(T)
P4HA1 Potri.017G075300 Prolyl 4-hydroxylase alpha subunit, hydroxylation of proline residues, potentially in hydroxyproline-rich glycoproteins in the cell wall [64] OE + = 12(C), 12(T)
PFD2 Potri.008G153900 Probable Prefoldin 2 protein. A heterohexameric chaperon protein that binds to actin, tubulin, and other proteins, possibly affecting the cortical spindle [55] OE + + 12(C), 13(T)
RWA2 Potri.010G148500 Reduced wall acetylation 2, catalyzes O-acetylation of cell wall polysaccharides [50] OE + + 13(C), 12(T)
SHMT Potri.001G320400 Serine hydroxymethyltransferase, reversible conversion of Ser and tetrahydrofolate to Gly and 5,10-methylene tetrahydrofolate, providing a major 1-carbon source for the cell [65] OE + + 12(C), 12(T)
VND6 Potri.015G127400 Vascular-related NAC-domain Protein 6, transcription factors involved in xylem vessel differentiation [66] OE - - 12(C), 13(T)
  1. KD knockdown of gene expression using RNAi, OE overexpression of target gene using a constitutive promoter (UBIQUITIN3)
  2. aGene model name based on phytozome [67] version 3.0 of the Populus trichocarpa genome
  3. bSugar release [14] and yield refer to performance relative to controls in greenhouse trials prior to the field trial
  4. cThe number of replicates (ramets) included in the statistical analyses for TOP (T) and comparator (C) lines. For DUF266 and GAUT12 there were two comparator lines, which are listed separately