Table 1 Description of genes targeted in this study
From: Agronomic performance of Populus deltoides trees engineered for biofuel production
Name | Genea | Description | Typeb | Sugar Releaseb | Yieldb | Rametsc |
|---|---|---|---|---|---|---|
CAD | Potri.009G095800 | Cinnamyl alcohol dehydrogenase, catalyzes the formation of coniferyl or coumaryl alcohol from their respective aldehydes [58] | KD | + | + | 12(C), 12(T) |
DUF231 | Potri.009G072800 | Domain of unknown function, a member of the Trichome Birefringence-Like (TBL) gene family, possibly responsible for O-acetylation of hemicelluloses [49] | OE | + | + | 12(C), 12(T) |
DUF266 | Potri.011G009500 | Domain of unknown function, possibly acting as a glycosyltransferase [53] | OE | + | + | 12(C), 5(C), 5(T) |
EPSPS | Potri.002G146400 | 5-enolpyruvylshikimate-3-phosphate synthase, key enzyme in biosynthesis of aromatic amino acids [59] | OE | + | = | 12(C), 12(T) |
EXT1;EXT2 | Potri.001G020100 Potri.005G190100 | Extensin, a basic hydroxyproline-rich glycoprotein localized to the cell wall [60] | KD | + | + | 12(C), 12(T); EXT2: 12(T) |
GAUT12 | Potri.001G416800 | Galacturonosyltransferase targeted to Golgi and involved in xylan and homogalacturonan biosynthesis [61] | KD | + | + | 12(C), 12(C), 12(T) |
HB3 | Potri.011G098300 | Transcription factor belonging to the HDZIPIII family, with high expression in xylem tissue [62] | KD | + | + | 12(C), 14(T) |
IQD10 | Potri.001G375700 | Calmodulin-binding protein with IQ amino acid-rich region with high expression in tension wood [63] | KD | + | + | 11(C), 13(T) |
P4HA1 | Potri.017G075300 | Prolyl 4-hydroxylase alpha subunit, hydroxylation of proline residues, potentially in hydroxyproline-rich glycoproteins in the cell wall [64] | OE | + | = | 12(C), 12(T) |
PFD2 | Potri.008G153900 | Probable Prefoldin 2 protein. A heterohexameric chaperon protein that binds to actin, tubulin, and other proteins, possibly affecting the cortical spindle [55] | OE | + | + | 12(C), 13(T) |
RWA2 | Potri.010G148500 | Reduced wall acetylation 2, catalyzes O-acetylation of cell wall polysaccharides [50] | OE | + | + | 13(C), 12(T) |
SHMT | Potri.001G320400 | Serine hydroxymethyltransferase, reversible conversion of Ser and tetrahydrofolate to Gly and 5,10-methylene tetrahydrofolate, providing a major 1-carbon source for the cell [65] | OE | + | + | 12(C), 12(T) |
VND6 | Potri.015G127400 | Vascular-related NAC-domain Protein 6, transcription factors involved in xylem vessel differentiation [66] | OE | - | - | 12(C), 13(T) |