Table 5 Analysis of consumed substrate and anaerobic metabolites after 44 h of cultivation of IAM1183-CDE/N and wild-type IAM1183 in 5-L fermenter
End product | IAM1183 | IAM1183-CDE/N | Change trend | Percent change (%) |
|---|---|---|---|---|
Glucose consumption (%) | 79.47 | 97.43 | ↑ | 22.26 |
Residual sugar (g/L) | 3.08 | 0.33 | ↓ | 87.34 |
Hydrogen (H2) (L) | 3.110 | 6.060 | ↑ | 94.85 |
Succinate (mM) | 1.02 | 3.16 | ↑ | 209.8 |
Lactate (mM) | 24.78 | 26.12 | ↑ | 5.400 |
Acetate (mM) | 15.33 | 11.47 | ↓ | 25.17 |
Formate (mM) | 1.260 | 0.790 | ↓ | 37.30 |
Ethanol (mM) | 10.54 | 12.28 | ↑ | 16.50 |
Citric acid (mM) | 1.930 | 1.750 | ↓ | 9.330 |
Acetoin (mM) | 20.73 | 18.58 | ↓ | 10.37 |
2,3-Butanediol (mM) | 6.380 | 7.740 | ↑ | 21.31 |