Fig. 2
Screening for and identification of novel regulators required for cellulase and xylanase production in P. oxalicum. a Venn diagram showing unique and shared candidate regulators selected on different carbon sources. The filtering criteria were |log2 fold change| ≤ 1.5, P ≤ 0.01, and probability ≥ 0.8. HP7-1_Glu, the HP7-1 strain grown in glucose medium; HP7-1_WB, the HP7-1 strain grown in wheat bran medium; HP7-1_WA, the HP7-1 strain grown in wheat bran plus Avicel medium. b Filter-paper cellulase activities of deletion mutants of the candidate regulatory genes on Avicel as the sole carbon source 6 days after inoculation. *P ≤ 0.05 and **P ≤ 0.01 between the deletion mutants and the parental strain ΔPoxKu70, assessed with Student’s t test. c Modular architecture of POX01167/PoxCxrA, POX04420/PoxCxrB, and POX08415/PoxNsdD. (D and E) Growth profiles of ΔPoxCxrA-6, ΔPoxCxrB-2, and ΔPoxNsdD-1 on glucose and Avicel, respectively. Data are the means of three biological replicates