Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria

Table 1 Strains used in this work

Name	ID	Species	Genotype	Accession number	References
M1354	LL345	Clostridium thermocellum	∆hpt	SRP053786	[34]
LL1048	LL1048		∆hfs::PgapD-cat	SRP108090	This work
LL1474	LL1474		∆hpt ∆hfsB	(Not sequenced)	This work
wt Tmat	LL1258	Thermoanaerobacter mathranii	wt	SRP108092	DSMZ 11426
LL1348	LL1348	Thermoanaerobacter mathranii	ΔhfsB::kan	SRP108103	This work
wt Tsac	LL1025	Thermoanaerobacterium saccharolyticum	wt	SRA234880	[35]
ALK2	LL1040		∆ldh::erm ∆(pta-ack)::kan	SRP084787	[18]
M1442	LL1049		∆(pta-ack) ∆ldh Δor795::metE-ure Δeps	SRP052455	[36]
M0353	LL1143		∆pyrF ∆(pta-ack) ∆ldh	SRP084629	[17]
HKO2	LL1186		Δech::erm	SRP085666	[19]
HKO3	LL1187		Δhfs::kan	SRP085668	[19]
HKO5	LL1188		Δech::erm Δhfs::kan	SRP086313	[19]
M2204	LL1189		hfs::hfs-kan*	SRP085677	[19]
HKO7	LL1190		Δhfs::kan ∆ldh::erm	SRP085674	[19]
HKO6	LL1191		Δhfs::kan ΔhydA::erm	SRP085673	[19]
M2205	LL1195		hfs::hfsDR107S-kan	SRP086210	[37]
LL1267	LL1267		ΔhfsA::kan	SRP108081	This work
LL1268	LL1268		ΔhfsB::kan	SRP108082	This work
LL1269	LL1269		ΔhfsC::kan	SRP108085	This work
LL1270	LL1270		ΔhfsD::kan	SRP108087	This work
LL1349	LL1349		ΔhfsAB::kan	SRP108105	This work
LL1350	LL1350		ΔhfsCD::kan	SRP108109	This work
wt Tthe	LL1244	Thermoanaerobacterium thermosaccharolyticum	wt	SRP096459	DSMZ 571
LL1346	LL1346	Thermoanaerobacterium thermosaccharolyticum	ΔhfsB::kan	SRP108099	This work
wt Txyl	LL1301	Thermoanaerobacterium xylanolyticum	wt	SRP108095	DSMZ 7097
LL1347	LL1347	Thermoanaerobacterium xylanolyticum	ΔhfsB::kan	SRP108102	This work

ISSN: 1754-6834