Fig. 3

a Stage 2 C. thermocellum CBP glucan plus xylan conversions and enzymatic hydrolysis glucan plus xylan yields for a cellulase loading of (b) 65 mg protein/g glucan in the pretreated biomass (c) and 15 mg protein/g glucan in the pretreated biomass as a function of time for Populus natural variants hydrothermally pretreated at a severity factor of 4.3 (200 °C and 22.7 min). Note: Protein loadings were based on glucan content in the pretreated biomass. Fermentations were sampled at 24, 48, 120, and 168 h and enzymatic hydrolysis reactions were sampled at 12, 24, 48, 120, and 168 h