Fig. 3From: Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33LC–MS profiles of the reaction catalyzed by recombinant Hno from A. tumefaciens S33. The HPLC profile was obtained by monitoring with a PDA detector at 210 nm; a–c the mass spectra of the substrate 6-hydroxynicotine (m/z 179.1) and the products 6-hydroxy-N-methylmyosmine (m/z 177.1) and 6-hydroxypseudooxynicotine (m/z 195.1), respectively. Positively charged ions were detectedBack to article page