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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33

Fig. 3

LC–MS profiles of the reaction catalyzed by recombinant Hno from A. tumefaciens S33. The HPLC profile was obtained by monitoring with a PDA detector at 210 nm; a–c the mass spectra of the substrate 6-hydroxynicotine (m/z 179.1) and the products 6-hydroxy-N-methylmyosmine (m/z 177.1) and 6-hydroxypseudooxynicotine (m/z 195.1), respectively. Positively charged ions were detected

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