Fig. 5From: Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33Biotransformation of nicotine into 6-hydroxynicotine catalyzed by the whole cells of A. tumefaciens S33-∆hno. a HPLC profile of the reaction mixtures sampled at different time after starting the reaction. The increase and decrease of the peaks during the reaction are indicated by upward or downward arrows, respectively. b Time course of the biotransformation reactionBack to article page