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Table 1 The NdhAB and Hno activity of wild-type A. tumefaciens S33, A. tumefaciens S33-∆hno, and A. tumefaciens S33-∆hno-C cultured in HSP medium (a) or nicotine–glucose–ammonium medium (b)

From: Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33

Enzyme Sp act (U/mg)-a Sp act (U/mg)-b
Wild-type S33 S33-∆hno S33-∆hno-C Wild-type S33 S33-∆hno S33-∆hno-C
NdhAB 0.47 ± 0.019 0.53 ± 0.021 0.52 ± 0.025 0.42 ± 0.018 0.03 ± 0.005 0.14 ± 0.013
Hno 0.05 ± 0.008 < 0.001 0.01 ± 0.002 0.07 ± 0.010 < 0.001 0.03 ± 0.007
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