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Table 1 The NdhAB and Hno activity of wild-type A. tumefaciens S33, A. tumefaciens S33-∆hno, and A. tumefaciens S33-∆hno-C cultured in HSP medium (a) or nicotine–glucose–ammonium medium (b)

From: Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33

Enzyme

Sp act (U/mg)-a

Sp act (U/mg)-b

Wild-type S33

S33-∆hno

S33-∆hno-C

Wild-type S33

S33-∆hno

S33-∆hno-C

NdhAB

0.47 ± 0.019

0.53 ± 0.021

0.52 ± 0.025

0.42 ± 0.018

0.03 ± 0.005

0.14 ± 0.013

Hno

0.05 ± 0.008

< 0.001

0.01 ± 0.002

0.07 ± 0.010

< 0.001

0.03 ± 0.007

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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