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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Laccase-derived lignin compounds boost cellulose oxidative enzymes AA9

Fig. 3

Enzymatic hydrolysis of Avicel incubated with supernatants rich in LMWLDC after LMS treatments of biomass at different conditions. The enzymatic hydrolysis was run with the LPMO containing cocktail Cellic® CTec2 (CT2). a In the y-axis are reported the cellulose (white bars) and glucose oxidation (gluconic acid, grey bars) conversion yield in percentage of the maximum theoretical cellulose conversion after 72 h at 50 °C. b The y-axis shows the percentage of the amount of gluconic acid over the amount of glucose hydrolyzed from cellulose both quantified as g/Kg. LPMOs oxidize the cellulose resulting in a non-reducing end and a C1-oxidized end. The subsequent actions of exo-cellulases and β-glucosidases are glucose and gluconic acid (monomer of C1 oxidation). Thus, the y-axis represents the percentage of the cellulose oxidized over the total amount cellulose hydrolyzed. Error bars represent the standard errors based on the means of triplicate experiments. CT2: control experiment with Avicel and CT2 enzymes only; CT2 + AA: Avicel, CT2 enzymes and ascorbic acid (AA); S (supernatants): CT2 + S1: Avicel, CT2 enzymes and supernatant containing LMWLDC from incubation of SCB and ABTS; CT2 + S2: Avicel, CT2 enzymes and LMWLDC from incubation of SCB and LMS-MtL; CT2 + S3: Avicel, CT2 enzymes and LMWLDC from incubation of SCB and LMS-TvL; CT2 + S4: Avicel, CT2 enzymes and LMWLDC from incubation of WS and ABTS; CT2 + S5: Avicel, CT2 enzymes and LMWLDC from incubation of WS and LMS-MtL; CT2 + S6: Avicel, CT2 enzymes and LMWLDC from incubation of WS and LMS-TvL

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