Fig. 3
From: Laccase-derived lignin compounds boost cellulose oxidative enzymes AA9
Enzymatic hydrolysis of Avicel incubated with supernatants rich in LMWLDC after LMS treatments of biomass at different conditions. The enzymatic hydrolysis was run with the LPMO containing cocktail Cellic® CTec2 (CT2). a In the y-axis are reported the cellulose (white bars) and glucose oxidation (gluconic acid, grey bars) conversion yield in percentage of the maximum theoretical cellulose conversion after 72 h at 50 °C. b The y-axis shows the percentage of the amount of gluconic acid over the amount of glucose hydrolyzed from cellulose both quantified as g/Kg. LPMOs oxidize the cellulose resulting in a non-reducing end and a C1-oxidized end. The subsequent actions of exo-cellulases and β-glucosidases are glucose and gluconic acid (monomer of C1 oxidation). Thus, the y-axis represents the percentage of the cellulose oxidized over the total amount cellulose hydrolyzed. Error bars represent the standard errors based on the means of triplicate experiments. CT2: control experiment with Avicel and CT2 enzymes only; CT2 + AA: Avicel, CT2 enzymes and ascorbic acid (AA); S (supernatants): CT2 + S1: Avicel, CT2 enzymes and supernatant containing LMWLDC from incubation of SCB and ABTS; CT2 + S2: Avicel, CT2 enzymes and LMWLDC from incubation of SCB and LMS-MtL; CT2 + S3: Avicel, CT2 enzymes and LMWLDC from incubation of SCB and LMS-TvL; CT2 + S4: Avicel, CT2 enzymes and LMWLDC from incubation of WS and ABTS; CT2 + S5: Avicel, CT2 enzymes and LMWLDC from incubation of WS and LMS-MtL; CT2 + S6: Avicel, CT2 enzymes and LMWLDC from incubation of WS and LMS-TvL