Fig. 7

CLSM combined images showing the spatial distribution (X–Y and Y–Z planes) of Archaea (red) and Bacteria (green) cells identified by FISH in aggregates from the unamended control (a), silica sand-supplemented control (b), wheat bran biochar (c), wood biochar (d), and orchard biochar (e). Biochar particles, visualized by their reflection signal in the same microscopic field, appear gray. Each image is composed by 32–40 optical sections of the aggregate thickness every 0.4–0.5 μm