Fig. 4

C. churrovis supernatant possesses higher relative activity than cellulose precipitated cellulosome compared to other fungi. Both culture supernatants and cellulose precipitated enzyme fractions were assessed for their activity on carboxymethyl cellulose, xylan, and pectin. Across the substrates tested, C. churrovis consistently had the highest specific activity in the supernatant compared to the cellulosome, including significantly (P < 0.05) more than P. finnis and N. californiae on CMC and significantly (P < 0.05) more than P. finnis and A. robustus on Xylan. These results suggest greater importance of free enzymes in C. churrovis and reflect the diverse array of enzymes possessed by anaerobic fungi. Protein gels are displayed in (Additional file 1: Figure S4). Error bars represent standard deviation of three replicates. *Represents significance, which was determined using the student’s t test