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Table 2 List of metabolites with decreased content (µg/g FW sorbitol equivalents) in leaf tissues of 6-month-old greenhouse-grown OXPdDUF231A versus K-147 plants

From: Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus

Metabolite [retention time (min); key m/z]

OXPdDUF231A/K-147

OXPdDUF231A

K-147

Fold change

p value

Avg

sem

Avg

sem

Iminodiacetic acid

0.02

0.003

3.3

0.9

188.2

63.6

Asparagine

0.03

0.000

2.4

1.0

68.5

14.7

10.84 158 200 302

0.03

0.000

0.6

0.1

21.2

2.2

Lysine

0.03

0.003

2.3

1.0

90.7

29.9

Ornithine

0.04

0.000

50.1

35.3

1290.1

170.1

Rutin

0.04

0.000

0.5

0.4

12.8

2.6

Luteolin

0.05

0.004

0.6

0.1

11.4

3.9

17.80 501 559 471 Flavonoid

0.06

0.005

1.4

0.3

21.2

7.4

7.92 218 231

0.06

0.000

0.3

0.1

4.7

0.6

13.75 219 Coumaroyl glycoside

0.07

0.001

1.3

0.1

17.6

4.3

9.49 302 316 288 242 208

0.07

0.000

4.5

1.7

68.5

11.6

α-Keto-glutaric acid

0.08

0.000

9.7

2.3

114.7

14.5

1,6-Anhydroglucose

0.08

0.000

16.7

3.0

209.7

37.7

Oxalomalic acid

0.08

0.000

7.5

1.3

96.6

17.6

Glutamine

0.08

0.001

6.8

1.8

90.3

24.4

14.11 521 171 289 Glycoside

0.09

0.013

1.9

0.7

20.0

8.2

3-Amino-2-piperidone

0.09

0.007

3.7

3.1

39.8

12.9

10.58 315 330 200 172

0.09

0.000

1.3

0.9

14.3

2.0

10.15 368 353 271 242 184

0.09

0.000

6.4

2.4

71.1

10.9

8.99 242 257 99

0.10

0.003

2.2

0.7

22.0

6.7

  1. Metabolites were analyzed as trimethylsilyl derivatives by gas chromatography–mass spectrometry. Data are the average (avg) and standard error of the mean (sem) of six OXPdDUF231A plants (three plants from each of two independent transgenic lines) and three K-147 control plants. Unknown and partially identified metabolites are designated by retention time, key mass-to-charge (m/z) ratios, and identified moieties