| |
Glycosyl residue composition of cell wall fractions (mol% ± SE)
|
|---|
|
Ara
|
Rha
|
Fuc
|
Xyl
|
GlcA
|
GalA
|
Man
|
Gal
|
Glc
|
|---|
|
Ammonium oxalate
|
|
WT
|
19.5 ± 0.8
|
5.1 ± 0.2
|
0.7 ± 0.02
|
8.4 ± 0.3
|
0.7 ± 0.01
|
23.6 ± 0.6
|
11.8 ± 0.7
|
4.5 ± 0.2
|
25.7 ± 1.3
|
|
V Control-1
|
18.9 ± 0.9
|
4.8 ± 0.1
|
0.7 ± 0.03
|
8.5 ± 0.3
|
0.7 ± 0.01
|
23.4 ± 0.7
|
12.6 ± 0.6
|
4.1 ± 0.2
|
26.3 ± 1.2
|
|
AB29.2
|
17.6 ± 0.9
|
5.3 ± 0.2
|
0.6 ± 0.02
|
10.2 ± 0.2*
|
0.7 ± 0.01
|
26.5 ± 0.8*
|
11.9 ± 0.4
|
5.1 ± 0.1*
|
22.5 ± 1.1*
|
|
AB29.7
|
18.1 ± 0.5
|
5.6 ± 0.1*
|
0.6 ± 0.02
|
10.5 ± 0.3*
|
0.6 ± 0.01
|
27.4 ± 0.9*
|
12.1 ± 0.6
|
4.8 ± 0.2
|
20.2 ± 0.9*
|
|
AB29.12
|
17.9 ± 0.6
|
5.4 ± 0.2
|
0.7 ± 0.03
|
10.7 ± 0.4**
|
0.7 ± 0.01
|
28.1 ± 0.5*
|
12.2 ± 0.5
|
4.7 ± 0.1
|
19.6 ± 1.1*
|
|
Sodium carbonate
|
|
WT
|
11.3 ± 0.6
|
5.8 ± 0.2
|
0.3 ± 0.03
|
22.4 ± 0.5
|
1.6 ± 0.06
|
18.9 ± 0.5
|
15.1 ± 0.6
|
6.8 ± 0.2
|
17.8 ± 0.8
|
|
V Control-1
|
11.4 ± 0.5
|
6.0 ± 0.3
|
0.2 ± 0.03
|
22.3 ± 0.5
|
1.3 ± 0.05
|
18.7 ± 0.6
|
15.3 ± 0.5
|
6.5 ± 0.3
|
18.3 ± 0.7
|
|
AB29.2
|
8.3 ± 0.5*
|
5.1 ± 0.3
|
0.4 ± 0.04
|
26.3 ± 0.3*
|
2.3 ± 0.06
|
22.9 ± 0.4*
|
14.6 ± 0.4
|
5.2 ± 0.2*
|
14.9 ± 0.6*
|
|
AB29.7
|
8.1 ± 0.4*
|
5.5 ± 0.2
|
0.3 ± 0.03
|
26.9 ± 0.4*
|
2.4 ± 0.05
|
23.6 ± 0.7*
|
14.1 ± 0.6
|
4.9 ± 0.2*
|
14.2 ± 0.7*
|
|
AB29.12
|
7.8 ± 0.6*
|
5.4 ± 0.3
|
0.5 ± 0.02
|
27.6 ± 0.6*
|
2.5 ± 0.06
|
25.7 ± 0.6*
|
13.5 ± 0.5*
|
4.7 ± 0.3*
|
12.3 ± 0.8*
|
|
1 M KOH
|
|
WT
|
0.3 ± 0.01
|
3.7 ± 0.1
|
0.2 ± 0.01
|
69.9 ± 1.3
|
8.7 ± 0.2
|
3.2 ± 0.1
|
5.9 ± 0.2
|
1.4 ± 0.08
|
6.7 ± 0.2
|
|
V Control-1
|
0.2 ± 0.01
|
3.5 ± 0.1
|
0.2 ± 0.01
|
70.0 ± 1.5
|
8.7 ± 0.3
|
3.1 ± 0.1
|
6.0 ± 0.3
|
1.6 ± 0.05
|
6.7 ± 0.4
|
|
AB29.2
|
0.3 ± 0.02
|
1.1 ± 0.1*
|
0.3 ± 0.01
|
79.8 ± 2.1*
|
9.9 ± 0.1*
|
3.6 ± 0.05*
|
2.3 ± 0.3*
|
0.9 ± 0.06**
|
1.8 ± 0.3**
|
|
AB29.7
|
0.2 ± 0.01
|
1.2 ± 0.3*
|
0.2 ± 0.01
|
81.1 ± 2.7*
|
9.9 ± 0.2*
|
3.7 ± 0.06*
|
1.1 ± 0.2*
|
0.6 ± 0.09**
|
2.0 ± 0.4**
|
|
AB29.12
|
0.2 ± 0.01
|
0.7 ± 0.3*
|
0.1 ± 0.01
|
82.2 ± 2.2**
|
10.0 ± 0.2*
|
3.9 ± 0.06**
|
1.2 ± 0.4*
|
0.2 ± 0.06**
|
1.5 ± 0.5**
|
|
4 M KOH
|
|
WT
|
2.1 ± 0.09
|
3.2 ± 0.1
|
0.3 ± 0.02
|
39.1 ± 1.4
|
4.7 ± 0.2
|
0.9 ± 0.03
|
10.8 ± 0.5
|
6.6 ± 0.2
|
32.3 ± 1.6
|
|
V Control-1
|
2.1 ± 0.03
|
3.1 ± 0.1
|
0.4 ± 0.02
|
39.0 ± 1.1
|
4.8 ± 0.1
|
0.9 ± 0.03
|
11.0 ± 0.3
|
6.9 ± 0.3
|
31.8 ± 1.7
|
|
AB29.2
|
2.2 ± 0.06
|
3.6 ± 0.08
|
0.5 ± 0.02
|
46.7 ± 1.3*
|
5.7 ± 0.2*
|
1.2 ± 0.05*
|
10.5 ± 0.6**
|
4.9 ± 0.4
|
24.7 ± 1.5*
|
|
AB29.7
|
1.8 ± 0.05
|
3.4 ± 0.1
|
0.4 ± 0.01
|
44.3 ± 1.1*
|
5.4 ± 0.1*
|
1.1 ± 0.03*
|
9.6 ± 0.3*
|
5.8 ± 0.6**
|
28.2 ± 1.4*
|
|
AB29.12
|
2.0 ± 0.06
|
3.8 ± 0.09*
|
0.3 ± 0.01
|
49.8 ± 1.5**
|
6.1 ± 0.2*
|
1.2 ± 0.03**
|
8.7 ± 0.5**
|
4.8 ± 0.3*
|
23.3 ± 1.6**
|
|
Sodium chlorite
|
|
WT
|
6.7 ± 0.3
|
3.9 ± 0.2
|
0.1 ± 0.01
|
11.6 ± 0.4
|
0.9 ± 0.03
|
7.8 ± 0.2
|
2.0 ± 0.04
|
11.7 ± 0.3
|
55.4 ± 1.5
|
|
V Control-1
|
6.9 ± 0.4
|
4.1 ± 0.2
|
0.2 ± 0.01
|
11.4 ± 0.6
|
0.8 ± 0.04
|
6.9 ± 0.2
|
1.9 ± 0.06
|
11.8 ± 0.4
|
56.0 ± 1.4
|
|
AB29.2
|
5.8 ± 0.4*
|
5.1 ± 0.1*
|
–
|
14.5 ± 0.4*
|
1.1 ± 0.03
|
8.9 ± 0.2*
|
1.7 ± 0.08*
|
11.6 ± 0.5
|
51.3 ± 1.1
|
|
AB29.7
|
6.1 ± 0.2*
|
4.3 ± 0.1*
|
–
|
13.3 ± 0.3*
|
1.0 ± 0.02
|
8.6 ± 0.1*
|
1.5 ± 0.09*
|
12.3 ± 0.4
|
52.9 ± 1.3
|
|
AB29.12
|
6.2 ± 0.3*
|
5.0 ± 0.2*
|
–
|
15.4 ± 0.5**
|
1.3 ± 0.05
|
10.1 ± 0.3*
|
1.4 ± 0.1**
|
11.1 ± 0.6
|
49.5 ± 0.9*
|
|
4 M KOH PC
|
|
WT
|
0.9 ± 0.03
|
2.2 ± 0.06
|
0
|
66.4 ± 1.9
|
8.2 ± 0.3
|
2.1 ± 0.09
|
6.0 ± 0.2
|
4.7 ± 0.3
|
9.5 ± 0.6
|
|
V Control-1
|
0.9 ± 0.05
|
2.3 ± 0.07
|
0
|
66.0 ± 2.6
|
8.1 ± 0.4
|
2.0 ± 0.10
|
5.9 ± 0.3
|
5.0 ± 0.3
|
9.8 ± 0.3
|
|
AB29.2
|
1.0 ± 0.03
|
2.5 ± 0.06*
|
0
|
78.3 ± 1.3*
|
9.6 ± 0.3*
|
2.7 ± 0.04*
|
1.9 ± 0.3**
|
1.3 ± 0.2*
|
2.7 ± 0.3**
|
|
AB29.7
|
0.9 ± 0.04
|
2.4 ± 0.08*
|
0
|
75.1 ± 1.2*
|
9.4 ± 0.2*
|
2.5 ± 0.05*
|
3.5 ± 0.2*
|
1.6 ± 0.3**
|
4.6 ± 0.4*
|
|
AB29.12
|
0.6 ± 0.06
|
2.3 ± 0.07
|
0
|
80.8 ± 1.5**
|
9.8 ± 0.4**
|
2.8 ± 0.07**
|
1.0 ± 0.4**
|
0.8 ± 0.2**
|
1.9 ± 0.2**
|
|
Insoluble
|
|
WT
|
3.9 ± 0.3
|
2.8 ± 0.1
|
0.2 ± 0.02
|
14.9 ± 0.3
|
0.2 ± 0.03
|
3.0 ± 0.4
|
9.5 ± 0.3
|
5.6 ± 0.1
|
59.9 ± 0.9
|
|
V Control-1
|
4.0 ± 0.1
|
3.1 ± 0.1
|
0.3 ± 0.03
|
15.3 ± 0.2
|
0.2 ± 0.04
|
2.9 ± 0.6
|
9.9 ± 0.2
|
5.4 ± 0.3
|
58.9 ± 0.8
|
|
AB29.2
|
3.9 ± 0.3
|
3.5 ± 0.1**
|
0.2 ± 0.02
|
16.0 ± 0.4*
|
0.2 ± 0.02
|
4.9 ± 0.3**
|
5.9 ± 0.3**
|
1.8 ± 0.2**
|
63.6 ± 0.7
|
|
AB29.7
|
3.8 ± 0.3
|
3.8 ± 0.09**
|
0.3 ± 0.02
|
16.4 ± 0.3*
|
0.3 ± 0.01
|
4.7 ± 0.5**
|
4.7 ± 0.4**
|
1.7 ± 0.3**
|
64.3 ± 0.6*
|
|
AB29.12
|
3.8 ± 0.3
|
4.1 ± 0.1**
|
0.3 ± 0.03
|
17.0 ± 0.5*
|
0.2 ± 0.02
|
5.5 ± 0.4**
|
3.5 ± 0.4**
|
1.4 ± 0.2**
|
64.2 ± 0.7*
|
- Alcohol insoluble residue (AIR) was sequentially extracted using ammonium oxalate, sodium carbonate, 1 M KOH, 4 M KOH, sodium chlorite (chlorite), and 4 M KOH post-chlorite (PC). Each fraction was then analyzed by GC–MS of tetramethylsilane (TMS) derivatives. The amounts of sugar are average mol% of wall extracts ± SE of 3 biological and 2 technical replicates (n = 6). Italics numbers with stars are mutant values that are significantly different from WT at * P ≤ 0.05, ** P ≤ 0.001 significant level (one-way ANOVA followed by Tukey’s multiple comparison test). A dash indicates that the sugar level was below detection limits
