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Table 3 Glycosyl linkage analysis of cell wall fractions from stems of P. deltoides WT and PtGAUT12.1-OE lines

From: Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

 

Ammonium oxalate soluble

Sodium carbonate soluble

1 M KOH soluble

WT

AB29.2

AB29.12

WT

AB29.2

AB29.12

WT

AB29.2

AB29.12

t-Araf

3.6

3.7

3.7

3.5

3.1

2.5

0.2

0.2

0.2

t-Arap

0.2

0.2

0.2

0.2

0.2

0.2

0.1

0.1

0.1

3-Araf

0.5

0.7

0.8

0.5

0.4

0.4

–

–

–

4-Arap or 5-Araf

3.2

1.9

1.8

2.1

1.0

1.1

0.2

0.1

1.1

3,4-Arap or 3,5-Araf

1.5

–

1.5

–

–

–

–

–

–

t-Rhap

0.9

0.9

0.9

1.9

1.1

1.3

0.2

0.2

0.2

2-Rhap

2.2

2.4

2.5

2.9

3.1

3.3

0.7

0.4

1.1

3-Rhap

–

–

–

–

–

–

1.6

2.0

2.2

2,4-Rhap

1.1

0.9

1.3

1.1

1.3

1.4

0.5

0.3

0.2

t-Fucp

0.3

0.5

0.3

0.3

0.4

0.8

0.2

0.3

0.2

t-Xylp

0.4

0.6

0.7

1.5

1.8

1.9

0.8

0.9

0.9

4-Xylp

6.5

7.9

7.6

20.1

23.7

24.2

57.8

66.5

69.4

2,4-Xylp

0.9

1.0

1.1

1.5

1.7

1.8

8.8

5.4

3.5

t-Manp

1.2

1.7

1.6

1.8

1.4

1.0

–

–

–

4-Manp

17.1

18.5

17.2

19.6

18.8

17.3

6.4

4.7

2.9

4,6-Manp

0.7

–

0.3

–

–

–

–

–

–

t-GlcAp

–

–

–

1.8

2.7

2.9

7.5

8.3

8.6

2-GlcAp

0.1

0.1

0.1

0.2

0.3

0.4

–

–

–

t-GalAp

1.1

1.9

1.9

2.0

3.1

3.3

–

1.1

1.3

2-GalAp

–

–

–

0.9

1.2

1.3

1.8

1.9

2.1

4-GalAp

23.8

26.8

27.5

16.1

19.9

20.8

1.5

4.7

4.9

3,4-GalAp

–

–

–

0.4

0.7

0.8

–

–

–

2,4-GalAp

0.1

0.3

0.4

0.1

–

–

–

–

–

t-Galp

2.9

2.6

2.8

3.2

2.1

2.0

1.2

0.3

0.1

2-Galp

0.4

–

0.4

0.6

–

–

–

–

–

4-Galp

–

–

–

–

–

–

–

–

–

6-Galp

0.4

0.7

0.4

–

–

–

0.5

0.6

0.2

3,4-Galp

–

1.7

0.4

0.3

–

–

–

–

–

2,4-Galp

0.2

0.2

0.2

0.2

–

–

–

–

–

t-Glcp

3.3

3.6

3.9

3.6

1.9

2.5

–

–

–

2-Glcp

0.3

0.1

0.2

0.1

0.2

0.1

–

–

–

4-Glcp

26.7

21.1

20.0

13.5

9.9

8.7

9.8

2.0

0.8

4,6-Glcp

0.4

–

0.6

–

–

–

–

–

–

  1. All numbers are mol percentages. A dash designates sugar level below detection limits. Glycosyl linkage analysis data were provided by CCRC Analytical Services