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Table 3 Glycosyl linkage analysis of cell wall fractions from stems of P. deltoides WT and PtGAUT12.1-OE lines

From: Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

  Ammonium oxalate soluble Sodium carbonate soluble 1 M KOH soluble
WT AB29.2 AB29.12 WT AB29.2 AB29.12 WT AB29.2 AB29.12
t-Araf 3.6 3.7 3.7 3.5 3.1 2.5 0.2 0.2 0.2
t-Arap 0.2 0.2 0.2 0.2 0.2 0.2 0.1 0.1 0.1
3-Araf 0.5 0.7 0.8 0.5 0.4 0.4
4-Arap or 5-Araf 3.2 1.9 1.8 2.1 1.0 1.1 0.2 0.1 1.1
3,4-Arap or 3,5-Araf 1.5 1.5
t-Rhap 0.9 0.9 0.9 1.9 1.1 1.3 0.2 0.2 0.2
2-Rhap 2.2 2.4 2.5 2.9 3.1 3.3 0.7 0.4 1.1
3-Rhap 1.6 2.0 2.2
2,4-Rhap 1.1 0.9 1.3 1.1 1.3 1.4 0.5 0.3 0.2
t-Fucp 0.3 0.5 0.3 0.3 0.4 0.8 0.2 0.3 0.2
t-Xylp 0.4 0.6 0.7 1.5 1.8 1.9 0.8 0.9 0.9
4-Xylp 6.5 7.9 7.6 20.1 23.7 24.2 57.8 66.5 69.4
2,4-Xylp 0.9 1.0 1.1 1.5 1.7 1.8 8.8 5.4 3.5
t-Manp 1.2 1.7 1.6 1.8 1.4 1.0
4-Manp 17.1 18.5 17.2 19.6 18.8 17.3 6.4 4.7 2.9
4,6-Manp 0.7 0.3
t-GlcAp 1.8 2.7 2.9 7.5 8.3 8.6
2-GlcAp 0.1 0.1 0.1 0.2 0.3 0.4
t-GalAp 1.1 1.9 1.9 2.0 3.1 3.3 1.1 1.3
2-GalAp 0.9 1.2 1.3 1.8 1.9 2.1
4-GalAp 23.8 26.8 27.5 16.1 19.9 20.8 1.5 4.7 4.9
3,4-GalAp 0.4 0.7 0.8
2,4-GalAp 0.1 0.3 0.4 0.1
t-Galp 2.9 2.6 2.8 3.2 2.1 2.0 1.2 0.3 0.1
2-Galp 0.4 0.4 0.6
4-Galp
6-Galp 0.4 0.7 0.4 0.5 0.6 0.2
3,4-Galp 1.7 0.4 0.3
2,4-Galp 0.2 0.2 0.2 0.2
t-Glcp 3.3 3.6 3.9 3.6 1.9 2.5
2-Glcp 0.3 0.1 0.2 0.1 0.2 0.1
4-Glcp 26.7 21.1 20.0 13.5 9.9 8.7 9.8 2.0 0.8
4,6-Glcp 0.4 0.6
  1. All numbers are mol percentages. A dash designates sugar level below detection limits. Glycosyl linkage analysis data were provided by CCRC Analytical Services