Fig. 4

Performance assay on pretreated corn stover (PCS). Synergistic conversion of PCS (5.0 g glucan/L) to soluble sugar at 40 °C and pH 5.0 was monitored using full-length GH7 CBH enzymes (~ 2.5 µM), together with a GH7 endoglucanase and a β-glucosidase (28, 1.9, and 0.5 mg enzyme per gram glucan, respectively). In addition to the three Trichoderma CBHs, Cel7A from Scytalidium sp. (ScyCel7A; identical to the enzyme called Geotrichum candidum Cel7A in [3]) and Cel7D from Phanerochaete chrysosporium were also analyzed at the same time; the results are shown for comparison. Experiments were performed in duplicate