Enzyme | kcat (s−1) | KM (mM) | kcat/KM (s−1 M−1) | Ki (µM) |
---|
TreCel7A_CD | 0.057 | 0.72 | 79 | 24 |
TatCel7A_CD | 0.019 | 1.00 | 19 | 72 |
ThaCel7A_CD | 0.067 | 0.90 | 75 | 49 |
- Ki is the competitive inhibition constant with 100 µM cellobiose in the reactions. The RMSD between calculated and experimental reaction rates was 3.4, 4.0, and 2.4% for TreCel7A_CD, TatCel7A_CD, and ThaCel7A_CD, respectively