Fig. 2
From: High activity CAZyme cassette for improving biomass degradation in thermophiles
Plasmid maps of Caldicellulosiruptor bescii expression vector for a Cbes_1867 (WT; GH9-CBM3c-CBM3b-CBM3b-GH48), b Cbes_1857 (GH10-CBM3b-CBM3b-GH48), c Cbes_1859 (GH5-CBM3b-CBM3b-GH44), and d Cbes_1865 (GH9-CBM3c-CBM3b-CBM3b-GH5) expression. Various multi-domain cellulases derived from C. bescii were expressed under the control of the regulatory region of the C. bescii S-layer protein. The expression vectors contain a signal peptide sequence, a C-terminal 6X His-tag, a Rho independent terminator, the pyrF (from C. thermocellum) cassette for selection, and pBAS2 sequences for replication in C. bescii. The apramycin resistant gene cassette (AprR), pSC101 low copy replication origin in E. coli, and repA, a plasmid-encoded gene required for pSC101 replication are indicated