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Table 2 Effects of inoculation OD, nitrogen source, soluble substrate, and fed-batch mode on the lipid fermentation of lignin stream by Rhodococcus opacus PD630

From: Combinatorial pretreatment and fermentation optimization enabled a record yield on lignin bioconversion

Parameters Effects of OD Effects of nitrogen source Effects of soluble substrates Effects of fermentation mode
Inoculum density (OD) 0.5, 1.0, 4.0, 8.0 1.0 1.0 1.0
(NH4)2SO4 (g/l) 1.4 0, 0.7,1.4, 2.1,2.8 1.4 1.4
Soluble substrate (g/l) 15 15 7.5, 15, 30, 45 45
Fermentation mode Batch Batch Batch Batch: 45 g/l (0 h)
     Fed-batch 1: 15 g/l (0 h) + 30 g/l (72 h)
     Fed-batch 2: 15 g/l (0 h) + 15 g/l (72 h) + 15 g/l (144 h)
  1. Lignin stream used in the fermentation optimization was produced from NaOH pretreatment (Case 1, as shown in Table 1)