Table 2 Effects of inoculation OD, nitrogen source, soluble substrate, and fed-batch mode on the lipid fermentation of lignin stream by Rhodococcus opacus PD630

Inoculum density (OD)

0.5, 1.0, 4.0, 8.0

1.0

1.0

1.0

(NH₄)₂SO₄ (g/l)

1.4

0, 0.7,1.4, 2.1,2.8

1.4

1.4

Soluble substrate (g/l)

15

15

7.5, 15, 30, 45

45

Fermentation mode

Batch

Batch

Batch

Batch: 45 g/l (0 h)

Fed-batch 1: 15 g/l (0 h) + 30 g/l (72 h)

Fed-batch 2: 15 g/l (0 h) + 15 g/l (72 h) + 15 g/l (144 h)