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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Processivity and enzymatic mechanism of a multifunctional family 5 endoglucanase from Bacillus subtilis BS-5 with potential applications in the saccharification of cellulosic substrates

Fig. 1

a SDS-PAGE analysis of cellulose-binding proteins secreted by B. subtilis BS-5. Lane M marker, Lane 1 fermentation broth of B. subtilis BS-5, Lane 2 unbound fractions in the fermentation broth after Avicel adsorption, Lane 3 cellulose-binding proteins bound to Avicel, eluted with SDS-PAGE buffer. b SDS-PAGE analysis of recombinant EG5C, EG5C-1 and EG5C-2 produced by E. coli. Lane M-marker, Lane 1-crude extracts of BL21-pET28a, Lanes 2, 4 and 6-crude extracts of E-pET-eg5c-1, E-pET-eg5c, E-pET-eg5c-2, Lines 3, 5 and 7-purified EG5C-1, EG5C and EG5C-2 with a HisTrap FF column

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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