Table 2 Fatty acid content of TAG in the recombinant P. tricornutum
Fatty acid | Nitrogen repletion | Nitrogen depletion for 10Â days | ||
|---|---|---|---|---|
Wild type (% of TAG) | PtWS/DGAToe (% of TAG) | Wild type (% of TAG) | PtWS/DGAToe (% of TAG) | |
Saturated fatty acid | ||||
 C14:0 | 10.73 ± 0.83 | 11.07 ± 0.64 | 6.27 ± 0.23 | 7.36 ± 0.58 |
 C15:0 | 0.59 ± 0.05 | 0.72 ± 0.07 | 0.52 ± 0.03 | 0.46 ± 0.04 |
 C16:0 | 32.84 ± 2.45 | 34.47 ± 3.49 | 40.04 ± 3.39 | 43.82 ± 2.93 |
 C18:0 | 7.31 ± 0.34 | 8.01 ± 0.48 | 3.07 ± 0.04 | 5.31 ± 0.21 |
 C19:0 | 1.26 ± 0.21 | 1.71 ± 0.30 | 0.72 ± 0.01 | 0.84 ± 0.03 |
 C20:0 | 0.31 ± 0.02 | 0.39 ± 0.03 | 0.19 ± 0.01 | 0.23 ± 0.01 |
 C22:0 | 0.83 ± 0.07 | 0.81 ± 0.03 | 0.57 ± 0.03 | 0.52 ± 0.05 |
 C24:0 | 3.73 ± 0.39 | 4.01 ± 0.23 | 3.24 ± 0.27 | 2.12 ± 0.72 |
Monounsaturated fatty acid | ||||
 C16:1 n − 1 | 29.56 ± 2.32 | 28.06 ± 3.74 | 36.85 ± 2.46 | 34.25 ± 2.93 |
 C16:1 n − 2 | 1.11 ± 0.32 | 0.73 ± 0.06 | 1.53 ± 0.17 | 1.20 ± 0.08 |
 C18:1 n − 1 | 2.11 ± 0.34 | 1.71 ± 0.25 | 4.43 ± 0.48 | 2.34 ± 0.40 |
 C18:1 n − 2 | 1.25 ± 0.04 | 1.27 ± 0.34 | 0.82 ± 0.02 | 0.17 ± 0.01 |
 C24:1 | 0.27 ± 0.01 | 0.28 ± 0.02 | 0.39 ± 0.03 | 0.13 ± 0.01 |
Polyunsaturated fatty acid | ||||
 C16:3 | 1.55 ± 0.06 | 1.04 ± 0.05 | 0.48 ± 0.03 | 0.53 ± 0.09 |
 C20:3 | 0.33 ± 0.02 | 0.13 ± 0.01 | 0.20 ± 0.01 | 0.15 ± 0.01 |
 C20:4 | 0.27 ± 0.01 | 0.21 ± 0.01 | 0.18 ± 0.01 | 0.20 ± 0.01 |
 C20:5 (EPA) | 5.86 ± 0.11 | 4.59 ± 0.07 | 0.46 ± 0.01 | 0.37 ± 0.01 |
 TAG (% of DW) | 3.85 ± 0.22 | 5.39 ± 0.15 (40%↑) | 18.27 ± 1.48 | 22.78 ± 1.59 (25%↑) |
 Total lipid (% of DW) | 11.25 ± 1.17 | 13.61 ± 1.84 (21%↑) | 32.64 ± 2.51 | 36.18 ± 2.38 (10%↑) |