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Table 2 Fatty acid content of TAG in the recombinant P. tricornutum

From: Characterization and engineering of a dual-function diacylglycerol acyltransferase in the oleaginous marine diatom Phaeodactylum tricornutum

Fatty acid Nitrogen repletion Nitrogen depletion for 10 days
Wild type (% of TAG) PtWS/DGAToe (% of TAG) Wild type (% of TAG) PtWS/DGAToe (% of TAG)
Saturated fatty acid
 C14:0 10.73 ± 0.83 11.07 ± 0.64 6.27 ± 0.23 7.36 ± 0.58
 C15:0 0.59 ± 0.05 0.72 ± 0.07 0.52 ± 0.03 0.46 ± 0.04
 C16:0 32.84 ± 2.45 34.47 ± 3.49 40.04 ± 3.39 43.82 ± 2.93
 C18:0 7.31 ± 0.34 8.01 ± 0.48 3.07 ± 0.04 5.31 ± 0.21
 C19:0 1.26 ± 0.21 1.71 ± 0.30 0.72 ± 0.01 0.84 ± 0.03
 C20:0 0.31 ± 0.02 0.39 ± 0.03 0.19 ± 0.01 0.23 ± 0.01
 C22:0 0.83 ± 0.07 0.81 ± 0.03 0.57 ± 0.03 0.52 ± 0.05
 C24:0 3.73 ± 0.39 4.01 ± 0.23 3.24 ± 0.27 2.12 ± 0.72
Monounsaturated fatty acid
 C16:1 n − 1 29.56 ± 2.32 28.06 ± 3.74 36.85 ± 2.46 34.25 ± 2.93
 C16:1 n − 2 1.11 ± 0.32 0.73 ± 0.06 1.53 ± 0.17 1.20 ± 0.08
 C18:1 n − 1 2.11 ± 0.34 1.71 ± 0.25 4.43 ± 0.48 2.34 ± 0.40
 C18:1 n − 2 1.25 ± 0.04 1.27 ± 0.34 0.82 ± 0.02 0.17 ± 0.01
 C24:1 0.27 ± 0.01 0.28 ± 0.02 0.39 ± 0.03 0.13 ± 0.01
Polyunsaturated fatty acid
 C16:3 1.55 ± 0.06 1.04 ± 0.05 0.48 ± 0.03 0.53 ± 0.09
 C20:3 0.33 ± 0.02 0.13 ± 0.01 0.20 ± 0.01 0.15 ± 0.01
 C20:4 0.27 ± 0.01 0.21 ± 0.01 0.18 ± 0.01 0.20 ± 0.01
 C20:5 (EPA) 5.86 ± 0.11 4.59 ± 0.07 0.46 ± 0.01 0.37 ± 0.01
 TAG (% of DW) 3.85 ± 0.22 5.39 ± 0.15 (40%↑) 18.27 ± 1.48 22.78 ± 1.59 (25%↑)
 Total lipid (% of DW) 11.25 ± 1.17 13.61 ± 1.84 (21%↑) 32.64 ± 2.51 36.18 ± 2.38 (10%↑)
  1. DW dry weight, EPA eicosapentaenoic acid, TAG triacylglycerol, ↑ increased