Fig. 3

Results of the β-Galactosidase activity measurement, qRT-PCR of glgC, glycogen quantitation and the nitrogen depletion assay. a β-Galactosidase activities in WT-LacZS, WT-LacZW, WT-PTLacZS, WT-PTLacZW, WT-HMLacZS, and WT-HMLacZW. The error bar represents the standard deviation of the three biological replicates for each sample. b qRT-PCR for the relative mRNA level of glgC in WT, WT-C, WT-HMC, WT-PTGLGC, and WT-HMGLGC. The error bar represents the standard deviation of the three technical replicates for each sample. c Glycogen quantitation assays in WT, WT-C, WT-HMC, WT-PTGLGC, and WT-HMGLGC. The error bar represents the standard deviation of the three biological replicates for each sample. d Nitrogen depletion assay in WT, WT-PTGLGC, and WT-HMGLGC