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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Fig. 3

Three-dimensional structure of CjGH5D in complex with XXXG-NHCOCH2Br and XyGOs. a Cartoon representation of the secondary structure of CjGH5D colour ramped from the N-terminus (blue) to the C-terminus (red). The two ligands XXXG-NHCOCH2Br and GXLG are overlaid in the active site cleft and shown in green and magenta sticks, respectively. b A close-up view of the active site cleft with the overlaid ligands XXXG-NHCOCH2Br in green and XXLG in magenta showing different amino acids interacting with the carbohydrate ligands. c 2Fo − Fc (σA/maximum likelihood weighted) electron density contoured in blue around GXLG in the CjGH5D-XXLG complex (left panel) and the chemical structure of the corresponding ligand (Right panel). Insufficient electron density was observed for the − 4′ xylosyl residue to allow modelling, therefore, it is shown in grey. d 2Fo − Fc electron density at 1σ (approx. 0.2 e3) contoured in blue around XXXG-NHCOCH2 moiety in the CjGH5D-XXXG-NHCOCH2Br complex (left panel) and chemical structure of the corresponding ligand (right panel). The bromide leaving group is shown in grey

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