Fig. 2
From: Function analysis of 5′-UTR of the cellulosomal xyl-doc cluster in Clostridium papyrosolvens
Characterization of the restriction profile by restriction assay. The plasmids (pMTC6) extracted from JM110 (Dam −, Dcm −) and Trans 5α (Dam +, Dcm +) were respectively incubated with C. papyrosolvens cell lysate in various NEB buffers for 20 h at 37 °C, using C. cellulolyticum cell lysate as a control. Lane 1 and 2: pMTC6 extracted from JM110 and DH5α; lane 3: C. cellulolyticum cell lysate; Lane 4 and 5: pMTC6 (Dam −, Dcm −) and pMTC6 (Dam +, Dcm +) incubated with C. cellulolyticum cell lysate; lane 6: C. papyrosolvens cell lysate; lane 7–11: pMTC6 (Dam −, Dcm −) incubated with C. papyrosolvens cell lysate in NEB buffer 1, 2, 3, 4, and Cutsmart buffer; lane 12–16: pMTC6 (Dam +, Dcm +) incubated with C. papyrosolvens cell lysate in various NEB buffers