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Table 1 The basic components of different treatments

From: Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii

Media Composition
TAPa Tris (2.42 g, 4× Beijerinck salts (25 mL), 1 M(K)PO4 (0.375 mL)
Trace (1 mL), acetate acids (1 mL), ddH2O (975 mL)
T-N NH4Cl in 4× Beijerinck salts change to KCl
T-N-P NH4Cl in 4× Beijerinck salts change to KCl, without adding 1 M(K)PO4
T-N+P NH4Cl in 4× Beijerinck salts change to KCl, adding two times 1 M(K)PO4
  1. aStock solutions for 1 L of TAP media: 1 M Tris base (e.g., Trizma) 20 mL, phosphate buffer II 1.0 mL solution A 10.0 mL, Hutner’s trace elements 1.0 mL, glacial acetic acid 1.0 mL, phosphate buffer II (for 100 mL) with K2HPO4 10.8 g, KH2PO4 5.6 g, solution A (for 500 mL) NH4Cl 20 g, MgSO4–7H2O 5 g, CaCl2–2H2O 2.5 g (adjust final pH to 7.0) [15]