Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii

Table 1 The basic components of different treatments

Media	Composition
TAP^a	Tris (2.42 g, 4× Beijerinck salts (25 mL), 1 M(K)PO₄ (0.375 mL) Trace (1 mL), acetate acids (1 mL), ddH₂O (975 mL)
T-N	NH₄Cl in 4× Beijerinck salts change to KCl
T-N-P	NH₄Cl in 4× Beijerinck salts change to KCl, without adding 1 M(K)PO₄
T-N+P	NH₄Cl in 4× Beijerinck salts change to KCl, adding two times 1 M(K)PO₄

^aStock solutions for 1 L of TAP media: 1 M Tris base (e.g., Trizma) 20 mL, phosphate buffer II 1.0 mL solution A 10.0 mL, Hutner’s trace elements 1.0 mL, glacial acetic acid 1.0 mL, phosphate buffer II (for 100 mL) with K₂HPO₄ 10.8 g, KH₂PO₄ 5.6 g, solution A (for 500 mL) NH₄Cl 20 g, MgSO₄–7H₂O 5 g, CaCl₂–2H₂O 2.5 g (adjust final pH to 7.0) [15]

ISSN: 2731-3654