The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive l-arabinose transport in Saccharomyces cerevisiae

Table 6 Comparison of key characteristics of Gal2, PcAraT and heterologous l-arabinose transporters that were previously expressed in S. cerevisiae

Protein	Origin	K_{m, ARA} [mM]	V_{max, ARA} [nmol (g biomass)⁻¹ min⁻¹]	GLC transport	XYL transport	Mechanism	References
ScGal2	S. cerevisiae	335 ± 21.0 57 ± 11 371 ± 19	75 ± 5 2.2 ± 0.3 18 ± 0.8	✓	✓	Facilitated diffusion	This study [19] [26]
PcAraT	P. chrysogenum	0.13 ± 0.03	5.3 ± 0.2	✗	✗	H⁺ symport	This study
SsAraT	Scheffersomyces stipitis	3.8 ± 1.7	0.4 ± 0.1	✓	✗	nd	[19]
AtStp2	Arabidopsis thaliana	4.5 ± 2.2	0.6 ± 0.1	✗	✗	H⁺ symport	[19]
KmAxt1	Kluyveromyces marxianus	263 ± 57	57 ± 6	✗	✓	Facilitated diffusion	[26]
PgAxt1	Pichia guilliermondii	0.13 ± 0.04	18 ± 0.8	✗	✓	H⁺ symport	[26]
AmLat1	Ambrosiozyma monospora	0.03*	0.2 ± 0.0	✗	✗	nd	[70, 73]
AmLat2	A. monospora	nd	4 ± 0	✗	✗	nd	[70, 73]
NcLat-1	Neurospora crassa	58 ± 4	1945 ± 50	✓	nd	H⁺ symport	[30]
MtLat-1	Myceliophthora. thermophila	29 ± 4	172 ± 6	✗	nd	H⁺ symport	[30]

nd, not determined; ARA, l-arabinose; GLC, d-glucose; XYL, d-xylose. * K_m of AmLat1 was determined as a GFP-fusion protein [73]

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