Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

Table 2 T ^app_m values of HMFO variants

T ^app_m values of HMFO variants (30 μM) obtained by ThermoFAD in phosphate buffer 50 mM pH 8.0
^aThe 7xHMFO variant includes the mutations: I73V, H74Y, G356H, V367L, T414K, A419Y, and A435E
^bThe 8AxHMFO variant includes the mutations: I73V, H74Y, G356H, V367L, T414K, A419Y, A435E, and W466F
^cThe 8BxHMFO variant includes the mutations: I73V, H74Y, G356H, V367R, T414K, A419Y, A435E, and W466F

ISSN: 2731-3654