HMFO | T
appm
(°C) |
---|
WT | 48.5 |
V367R W466F | 39.5 |
7xHMFOa | 60.5 |
8AxHMFOb | 55.5 |
8BxHMFOc | 51.5 |
- T
appm
values of HMFO variants (30 μM) obtained by ThermoFAD in phosphate buffer 50 mM pH 8.0
- aThe 7xHMFO variant includes the mutations: I73V, H74Y, G356H, V367L, T414K, A419Y, and A435E
- bThe 8AxHMFO variant includes the mutations: I73V, H74Y, G356H, V367L, T414K, A419Y, A435E, and W466F
- cThe 8BxHMFO variant includes the mutations: I73V, H74Y, G356H, V367R, T414K, A419Y, A435E, and W466F