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Table 2 T appm values of HMFO variants

From: Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

HMFO T appm (°C)
WT 48.5
V367R W466F 39.5
7xHMFOa 60.5
8AxHMFOb 55.5
8BxHMFOc 51.5
  1. T appm values of HMFO variants (30 μM) obtained by ThermoFAD in phosphate buffer 50 mM pH 8.0
  2. aThe 7xHMFO variant includes the mutations: I73V, H74Y, G356H, V367L, T414K, A419Y, and A435E
  3. bThe 8AxHMFO variant includes the mutations: I73V, H74Y, G356H, V367L, T414K, A419Y, A435E, and W466F
  4. cThe 8BxHMFO variant includes the mutations: I73V, H74Y, G356H, V367R, T414K, A419Y, A435E, and W466F