Fig. 7

Recovery study for NcLPMO9C concentration. Dark gray diamonds represent the found LPMO concentration plotted against the added LPMO concentration. The activity of different added NcLPMO9C concentrations was measured with the LPMO assay at 30 °C with 1 mM 2,6-DMP, 100 µM H₂O₂ in 100 mM succinate/phosphate buffer and the found LPMO concentration was calculated from the measured volumetric activity, the specific LPMO activity of 32.3 U g⁻¹, and the molecular mass of NcLPMO9C (34,300 g mol⁻¹). A linear range was observed up to a NcLPMO9C concentration of 1.25 µM/42.9 µg mL⁻¹. The inset shows the non-linear range with a significant deviation of the found LPMO volumetric activity or recalculated found LPMO concentration. The calculation of the fit is restricted to the linear range. The data are expressed as mean values (± SD), from four independent repeats