A fast and sensitive activity assay for lytic polysaccharide monooxygenase

Table 2 2,6-DMP conversion rates in control reactions at pH 7.5

Specific activities	U g⁻¹
NcLPMO9C + 2,6-DMP + H₂O₂	32.3 ± 0.9
NcLPMO9C (heat inactivated) + 2,6-DMP + H₂O₂	0.2 ± 0.1
NcLPMO9C + 2,6-DMP + H₂O₂ + 20 µM EDTA	0.4 ± 0.4
NcLPMO9C + 2,6-DMP (no H₂O₂ added)	0.6 ± 0.4
NcLPMO9C + H₂O₂ (no 2,6-DMP added)	0.1 ± 0.1
CuSO₄ + 2,6-DMP + H₂O₂	0.6 ± 0.3
CuSO₄ + 2,6-DMP (no H₂O₂ added)	0.5 ± 0.4
2,6-DMP + H₂O₂ (no NcLPMO9C added)	0.1 ± 0.1

Concentrations of catalysts/reactants were 0.5 µM NcLPMO9C, 0.5 µM CuSO₄, 1 mM 2,6-DMP, 100 µM H₂O₂

ISSN: 2731-3654