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Table 1 Main parameters used for the design of the recombinant enzyme production process

From: Techno-economic analysis of the industrial production of a low-cost enzyme using E. coli: the case of recombinant β-glucosidase

Parameter Assumption
Enzyme titer after primary recovery and concentration 15 g/L
Annual operating time of the enzyme production unit 7920 h (330 days)
Nominal volume of the main bioreactor 100 m3
Maximum working volume of the fermenter 80%
Temperature 26 °C
Overpressure 150 kPa
Fermenter material Stainless steel—grade 316 (SS316)
µ 0.23 h−1
pH 6.8
Glucose concentration (during fed-batch phase) 1.5 g/L
pO2 20%
Nominal volume of the main bioreactor 100 m3
Enzyme titer after primary recovery and concentration 15 g/L
Annual operating time of the enzyme production unit 7920 h (330 days)