Table 1 Main parameters used for the design of the recombinant enzyme production process
Parameter | Assumption |
|---|---|
Enzyme titer after primary recovery and concentration | 15 g/L |
Annual operating time of the enzyme production unit | 7920 h (330 days) |
Nominal volume of the main bioreactor | 100 m3 |
Maximum working volume of the fermenter | 80% |
Temperature | 26 °C |
Overpressure | 150 kPa |
Fermenter material | Stainless steel—grade 316 (SS316) |
µ | 0.23 h−1 |
pH | 6.8 |
Glucose concentration (during fed-batch phase) | 1.5 g/L |
pO2 | 20% |
Nominal volume of the main bioreactor | 100 m3 |
Enzyme titer after primary recovery and concentration | 15 g/L |
Annual operating time of the enzyme production unit | 7920 h (330 days) |