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Table 2 Effects of engineering individual genes on free octanoic acid production

From: Engineering of E. coli inherent fatty acid biosynthesis capacity to increase octanoic acid production

Strain Time (h) Cell density (OD550) Glucose used (g/L) Acetate titer (g/L) C8 titer (mg/L) C8 yield (mg/g glucose) C8 productivity (mg/L/h) Total FFAs (mg/L)
MG1655 (TE10) 72 1.91 ± 0.1 13.8 ± 0.2 0.63 ± 0.02 275 ± 12 19.9 ± 0.9 3.81 ± 0.17 315 ± 14
+fabB (TE10) 72 1.87 ± 0.06 13.4 ± 0.1 0.62 ± 0.02 250 ± 5.7 18.6 ± 0.4 3.46 ± 0.079 281 ± 5.7
+fabF (TE10) 72 1.72 ± 0.05 13.2 ± 0.3 0.65 ± 0.03 253 ± 6.4 19.2 ± 0.3 3.52 ± 0.088 292 ± 11
+fabG (TE10) 72 1.81 ± 0.1 13.9 ± 0.4 0.67 ± 0.05 293 ± 5.5 21.0 ± 0.4 4.07 ± 0.076 326 ± 28
+fabZ (TE10) 72 4.51 ± 0.2 14.8 ± 0.1 1.8 ± 0.2 398 ± 8.3 26.9 ± 0.6 5.53 ± 0.12 479 ± 22
+fabZGB (TE10) 72 5.74 ± 0.4 14.8 ± 0.1 0.52 ± 0.06 278 ± 13 18.8 ± 0.9 3.86 ± 0.18 403 ± 16
+fabZGF (TE10) 72 5.50 ± 0.2 14.6 ± 0.1 1.1 ± 0.2 207 ± 9.5 14.2 ± 0.7 2.88 ± 0.13 309 ± 11
ΔfadE (TE10) 72 2.20 ± 0.1 13.6 ± 0.4 1.2 ± 0.1 286 ± 8.8 21.1 ± 0.6 3.97 ± 0.12 333 ± 10
ΔfadB (TE10) 72 1.84 ± 0.1 13.3 ± 0.1 1.3 ± 0.1 270 ± 4.4 20.3 ± 0.3 3.75 ± 0.062 310 ± 5.2
ΔfadA (TE10) 72 1.84 ± 0.02 13.8 ± 0.2 1.4 ± 0.1 270 ± 3.5 19.5 ± 0.3 3.74 ± 0.048 309 ± 4.4
ΔfumAC (TE10) 72 1.73 ± 0.07 12.9 ± 0.3 1.4 ± 0.2 241 ± 6.5 18.7 ± 0.5 3.35 ± 0.048 277 ± 3.9
ΔackA (TE10) 72 2.06 ± 0.06 13.2 ± 0.1 0.6 ± 0.1 289 ± 5.7 21.9 ± 0.4 4.00 ± 0.079 330 ± 6.6
Δpta (TE10) 72 1.97 ± 0.03 12.9 ± 0.2 0.5 ± 0.1 284 ± 2.6 22.0 ± 0.2 3.94 ± 0.036 324 ± 3.4