Table 2 Effects of engineering individual genes on free octanoic acid production
Strain | Time (h) | Cell density (OD550) | Glucose used (g/L) | Acetate titer (g/L) | C8 titer (mg/L) | C8 yield (mg/g glucose) | C8 productivity (mg/L/h) | Total FFAs (mg/L) |
|---|---|---|---|---|---|---|---|---|
MG1655 (TE10) | 72 | 1.91 ± 0.1 | 13.8 ± 0.2 | 0.63 ± 0.02 | 275 ± 12 | 19.9 ± 0.9 | 3.81 ± 0.17 | 315 ± 14 |
+fabB (TE10) | 72 | 1.87 ± 0.06 | 13.4 ± 0.1 | 0.62 ± 0.02 | 250 ± 5.7 | 18.6 ± 0.4 | 3.46 ± 0.079 | 281 ± 5.7 |
+fabF (TE10) | 72 | 1.72 ± 0.05 | 13.2 ± 0.3 | 0.65 ± 0.03 | 253 ± 6.4 | 19.2 ± 0.3 | 3.52 ± 0.088 | 292 ± 11 |
+fabG (TE10) | 72 | 1.81 ± 0.1 | 13.9 ± 0.4 | 0.67 ± 0.05 | 293 ± 5.5 | 21.0 ± 0.4 | 4.07 ± 0.076 | 326 ± 28 |
+fabZ (TE10) | 72 | 4.51 ± 0.2 | 14.8 ± 0.1 | 1.8 ± 0.2 | 398 ± 8.3 | 26.9 ± 0.6 | 5.53 ± 0.12 | 479 ± 22 |
+fabZGB (TE10) | 72 | 5.74 ± 0.4 | 14.8 ± 0.1 | 0.52 ± 0.06 | 278 ± 13 | 18.8 ± 0.9 | 3.86 ± 0.18 | 403 ± 16 |
+fabZGF (TE10) | 72 | 5.50 ± 0.2 | 14.6 ± 0.1 | 1.1 ± 0.2 | 207 ± 9.5 | 14.2 ± 0.7 | 2.88 ± 0.13 | 309 ± 11 |
ΔfadE (TE10) | 72 | 2.20 ± 0.1 | 13.6 ± 0.4 | 1.2 ± 0.1 | 286 ± 8.8 | 21.1 ± 0.6 | 3.97 ± 0.12 | 333 ± 10 |
ΔfadB (TE10) | 72 | 1.84 ± 0.1 | 13.3 ± 0.1 | 1.3 ± 0.1 | 270 ± 4.4 | 20.3 ± 0.3 | 3.75 ± 0.062 | 310 ± 5.2 |
ΔfadA (TE10) | 72 | 1.84 ± 0.02 | 13.8 ± 0.2 | 1.4 ± 0.1 | 270 ± 3.5 | 19.5 ± 0.3 | 3.74 ± 0.048 | 309 ± 4.4 |
ΔfumAC (TE10) | 72 | 1.73 ± 0.07 | 12.9 ± 0.3 | 1.4 ± 0.2 | 241 ± 6.5 | 18.7 ± 0.5 | 3.35 ± 0.048 | 277 ± 3.9 |
ΔackA (TE10) | 72 | 2.06 ± 0.06 | 13.2 ± 0.1 | 0.6 ± 0.1 | 289 ± 5.7 | 21.9 ± 0.4 | 4.00 ± 0.079 | 330 ± 6.6 |
Δpta (TE10) | 72 | 1.97 ± 0.03 | 12.9 ± 0.2 | 0.5 ± 0.1 | 284 ± 2.6 | 22.0 ± 0.2 | 3.94 ± 0.036 | 324 ± 3.4 |