Table 4 Strains and plasmids used in this study
Strains/plasmids | Genetic characteristics | Source |
|---|---|---|
Strains | ||
 MG1655 | Wild-type E. coli K1-12 strain | Lab collection |
 +fadB | MG1655, mgsA::M1-93-fadB | This study |
 +fadF | MG1655, mgsA::M1-93-fadF | This study |
 +fadG | MG1655, mgsA::M1-93-fadG | This study |
 +fadZ | MG1655, mgsA::M1-93-fadZ | This study |
 +fadZGB | MG1655, mgsA::M1-93-fadZ-RBS1-fabG-RBS2-fabB | This study |
 +fadZGF | MG1655, mgsA::M1-93-fadZ-RBS1-fabG-RBS2-fabF | This study |
 ΔfadE | MG1655, ΔfadE | This study |
 ΔfadA | MG1655, ΔfadA | This study |
 ΔfadB | MG1655, ΔfadB | This study |
 ΔfumAC | MG1655, ΔfumAC | This study |
 ΔackA | MG1655, ΔackA | This study |
 Δpta | MG1655, Δpta | This study |
 +fadZ ΔfadE | MG1655, mgsA::M1-93-fadZ, ΔfadE | This study |
 +fadZ ΔfadA | MG1655, mgsA::M1-93-fadZ, ΔfadA | This study |
 +fadZ ΔfadB | MG1655, mgsA::M1-93-fadZ, ΔfadB | This study |
 +fadZ ΔfadE ΔfumAC | MG1655, mgsA::M1-93-fadZ, ΔfadE, ΔfumAC | This study |
 +fadZ ΔfadE ΔfumAC ΔackA (ZEFA) | MG1655, mgsA::M1-93-fadZ, ΔfadE, ΔfumAC, ΔackA | This study |
 +fadZ ΔfadE ΔfumAC Δpta | MG1655, mgsA::M1-93-fadZ, ΔfadE, ΔfumAC, Δpta | This study |
Plasmids | ||
 pJMYEEI82564 (TE10) | pTrc-EEI82564 thioesterase from Anaerococcus tetradius, Ampr | [49] |