Table 2 Primers and probes used in this study
From: A long noncoding RNA promotes cellulase expression in Trichoderma reesei
Name | Sequence (5′–3′) | Employment(s) |
|---|---|---|
act1f | TGAGAGCGGTGGTATCCACG | qPCR |
act1r | GGTACCACCAGACATGACAATGTTG | qPCR |
amdS inv for | CAAAGGAAGAATCCCTTCAGGGTTGCGTTTCCAG | Inverse PCR |
amdS inv rev | CGCAGTTGCGTGGGATGACATTCATACTCAAGAC | Inverse PCR |
hax1 for_down-Intron | CCAGCTCCAACAGAACCAGG | Sequencing |
hax for_inter-Intron | GCAATGTCGCCAGGCACCG | qPCR |
hax1 for_qPCR_QM9414 | GTTCAAGCCCGTTCAAGCCC | qPCR |
hax1 for_QM6a_BcuI | CAGCAGTACTAGTCCCACCGGCAGGTGGCTAAACGG | Qualitative PCR, cloning OEhax1 |
hax1 for_QM9414_BcuI | CAGCAGACTAGTGGTCAGGCCCGTTCAAGCCCGTTC | Qualitative PCR, cloning OEhax1 |
hax1 for_Rut-C30_BcuI | CAAGACTAGTGAAGTTCCACACGGATACAGAGACACAACATG | Qualitative PCR, cloning OEhax1 |
hax1 for_3 | TGCCTTGCATCGTACCGCTG | qPCR |
hax1 for_3′RACE_3 | AGGTGCTAAAACTGAATGGATGG | RACE |
hax1 rev | TCCGCAAAACGAACCGAACC | qPCR |
hax1 rev_down-Intron | GAGCGGCAGGAGGATGGATTC | qPCR |
hax1 rev_inter-Intron | GAGGTGCGCCGCGAATCTG | Sequencing |
hax1 rev kurz | TGAGTCGAGGGGCTACTGCAAGTAC | qPCR |
hax1 rev_up-Intron | GCCATCCATTCAGTTTTAGCACC | qPCR, RACE |
hax1 rev_1.Intron | CACGCATTTCATCTGGCCATTG | qPCR |
hax1 rev_3′QM6a | CACGCATTTCATCTGGCCATTGAGTATCTACG | Qualitative PCR, cloning OEhax1 |
hax1 rev_3-39_XbaI | CAGCAGTCTAGAGACTTCTTGGTATTGTCGTCACCTTATG | Sequencing |
hax1 rev_5′RACE_2 | CTACGATACACCGACTTCTTGG | RACE |
hax1 rev_ 5′RACE_4 | CGTCCCCACAATAGCCATGAGCCG | RACE |
Isopropylmalate synthase forward | TCCCGAATGCTTCTCCGACA | qPCR |
Isopropylmalatesynthase_rev | GCTCTCTCTGTCTCCGATGTTGG | qPCR |
Locus for | CGCGCTCTCTTTTCCTCCTT | Dhax1 candidate screening |
Locus rev | GCAGAACCCAGGACACAAAGAGC | Dhax1 candidate screening |
Locus 3′for Acc65I | GGTACCTGAGGTGTATGATTCCGTGATG | Cloning Dhax1 |
Locus 3′rev XbaI | TCTAGATCCTGTTCGGCCCAGCCC | Cloning Dhax1 |
Locus 5′for SalI | GGGAATAACCAATGCCTCTGAAGCTT | Cloning Dhax1 |
Locus 5′rev HindIII | GGGAATAACCAATGCCTCTGAAGCTT | Cloning Dhax1 |
Pbgl1 for_Kpn2I | CAAGTCCGGAGCAAGCGATAACCATAGGTA | Cloning OEhax1 |
Pbgl1 rev-NheI | CAACAAGCTAGCCTCAACAAAGCAGAGTCTTG | Cloning OEhax1 |
5pyr4_fwd(BglII) | GCGGAAGATCTCGAGATAGTATCTC | OEhax1 candidate screening |
sar1fw | TGGATCGTCAACTGGTTCTACGA | qPCR |
sar1rev | GCATGTGTAGCAACGTGGTCTTT | qPCR |
sonde hax_5-biotin | [biotin]CGTCAGCCACCAGCCACCGTTTAGCCACCT | HAX1 enrichment |
Unknown protein forward | CGCCGTATTGGGGTTCATTG | qPCR |
Unknown protein reverse | GGCTAAACGTTGTCTCGGAG | qPCR |
up-hax1 for_1 | ACATGCAGGAGATTGGGCGTC | qPCR |
up-hax1 for_2 | CGCGGCTTAATCAGAGGTGGG | qPCR |