Fig. 1

Experimental design and physiological assays. a Experimental design for repeated dehydration stresses and sample collection. b Experimental design for determining the persistence of dehydration memory and sample collection. c Survival rates of trained and non-trained switchgrass. d Relative water content of leaves during repeated dehydration stresses. e Water loss from leaves during the first, second and third dehydration stresses. f–i, Determination of photosynthetic activity during repeated dehydration stresses. C control, D1–3 the first, second, and third dehydration stresses, R1–2 the first and second recovery periods, RNA-Seq RNA sequencing, qRT-PCR quantitative real-time PCR, RWC relative water content, PS I and PS II photosynthesis system I and II, ABA abscisic acid, JA jasmonic acid, Fv/Fm maximum photosynthetic potential, qN non-photochemical quenching, Yield (I) and Yield (II) real-time quantum yield of PSI and PSII. Values for RWC, water loss and photosynthesis parameters are means of n = 5 measurements ± SD