Fig. 1

n-Hexanol tolerance of evolved strains. a Average specific growth rates (µ_avg) for wild-type (WT) and evolved strains grown in 10 ml YPD + 0.15% n-hexanol in 50 ml culture tubes were calculated from growth curves as described in “Methods”. Values are mean ± standard deviation from 9, 7, 4, 4, and 4 independent cultures of WT, sSD003, sSD006, sSD019, sSD021, respectively. b Biomass yield (OD₆₀₀) measured at 24 h for three independent cultures of WT and evolved strains in the presence of n-hexanol concentrations in the range 0–0.3%