Fig. 5

Amino acid substitutions of D85 of Gcd1p and D77 of Sui2p modulate medium-chain alcohol tolerance. Plasmids for the expression of mutant Gcd1p and Sui2p (Additional file 1: Table S2) were transformed into wild-type BY4741. Overnight cultures of strains were diluted to an OD₆₀₀ of 0.15 AU in 10 ml SD medium lacking uracil and containing either 0.13% n-hexanol (a, b), 0.5% n-pentanol (c, d), or 0.05% n-heptanol (e, f). Strains harboring the plasmids expressing Gcd1p mutants are shown in a, c, and e, while strains harboring the plasmids expressing Sui2p mutants are shown in b, d, and f. The μavg was calculated from growth curves for the time period between 4 and 12 h. The grey bar indicates the WT protein, the orange bar, the substitutions identified in the evolved strains. X represents the substituted amino-acid. Values are means ± standard deviation from three independent cultures