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Table 1 List of Saccharomyces cerevisiae strains and plasmids used in this study and their genotypes

From: Evolutionary engineering improves tolerance for medium-chain alcohols in Saccharomyces cerevisiae

Strain Genotype Plasmid Plasmid description Source
BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 None   John Duebera
sSD003 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1 - 1 cit2 - 1 None   This study
sSD006 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7 - 2 None   This study
sSD019 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1 - 1 cit2 - 1 pdr5 - 1 ubp13 - 1 lsb6 - 1 nst1 - 1 cox1 - 1 None   This study
sSD021 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7 - 2 sui2 - 2 pdr5 - 2 sey1 - 2 None   This study
sSD029 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1Δ::KanMX4 pSD450 pAH056 CEN6/ARS4 URA3 gcd1-1 This study
sSD040 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7Δ::His3 pSD469 pAH005 CEN6/ARS4 LEU2 gcd7-2 This study
sSD053 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1Δ::KanMX4 pSD442 pAH056 CEN6/ARS4 URA3 GCD1 This study
sSD054 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7Δ::His3 pSD474 pAH005 CEN6/ARS4 LEU2 GCD7 This study
sSD055 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 pSD444 pAH056 CEN6/ARS4 URA3 SUI2 This study
sSD056 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 pSD452 pAH056 CEN6/ARS4 URA3 sui2-2 This study
sSD058 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 gcd7Δ::His3 pSD452/pSD469 pAH056 CEN6/ARS4 URA3 sui2-2 pAH005 CEN6/ARS4 LEU2 gcd7-2 This study
sSD060 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 gcd7Δ::His3 pSD444/pSD474 pAH056 CEN6/ARS4 URA3 SUI2 pAH005 CEN6/ARS4 LEU2 GCD7 This study
sSD061 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 pSD442 pAH056 CEN6/ARS4 URA3 GCD1 This study
sSD062 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 pSD450 pAH056 CEN6/ARS4 URA3 gcd1-1 This study
sSD063 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 pSD444 pAH056 CEN6/ARS4 URA3 SUI2 This study
sSD064 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 pSD452 pAH056 CEN6/ARS4 URA3 sui2-2 This study
  1. Highlighted in bold are mutant alleles identified in the evolved strains
  2. aDept. of Bioengineering, University of California Berkeley