Table 1 List of Saccharomyces cerevisiae strains and plasmids used in this study and their genotypes
From: Evolutionary engineering improves tolerance for medium-chain alcohols in Saccharomyces cerevisiae
Strain | Genotype | Plasmid | Plasmid description | Source |
|---|---|---|---|---|
BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | None | John Duebera | |
sSD003 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1 - 1 cit2 - 1 | None | This study | |
sSD006 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7 - 2 | None | This study | |
sSD019 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1 - 1 cit2 - 1 pdr5 - 1 ubp13 - 1 lsb6 - 1 nst1 - 1 cox1 - 1 | None | This study | |
sSD021 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7 - 2 sui2 - 2 pdr5 - 2 sey1 - 2 | None | This study | |
sSD029 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1Δ::KanMX4 | pSD450 | pAH056 CEN6/ARS4 URA3 gcd1-1 | This study |
sSD040 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7Δ::His3 | pSD469 | pAH005 CEN6/ARS4 LEU2 gcd7-2 | This study |
sSD053 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd1Δ::KanMX4 | pSD442 | pAH056 CEN6/ARS4 URA3 GCD1 | This study |
sSD054 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 gcd7Δ::His3 | pSD474 | pAH005 CEN6/ARS4 LEU2 GCD7 | This study |
sSD055 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 | pSD444 | pAH056 CEN6/ARS4 URA3 SUI2 | This study |
sSD056 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 | pSD452 | pAH056 CEN6/ARS4 URA3 sui2-2 | This study |
sSD058 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 gcd7Δ::His3 | pSD452/pSD469 | pAH056 CEN6/ARS4 URA3 sui2-2 pAH005 CEN6/ARS4 LEU2 gcd7-2 | This study |
sSD060 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sui2Δ::KanMX4 gcd7Δ::His3 | pSD444/pSD474 | pAH056 CEN6/ARS4 URA3 SUI2 pAH005 CEN6/ARS4 LEU2 GCD7 | This study |
sSD061 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | pSD442 | pAH056 CEN6/ARS4 URA3 GCD1 | This study |
sSD062 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | pSD450 | pAH056 CEN6/ARS4 URA3 gcd1-1 | This study |
sSD063 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | pSD444 | pAH056 CEN6/ARS4 URA3 SUI2 | This study |
sSD064 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | pSD452 | pAH056 CEN6/ARS4 URA3 sui2-2 | This study |