Fig. 2
Batch cultivation of C. glutamicum I-pXMJ19 (squares), I-eco (diamonds), I-ppm (circles), I-sco (upward triangles), and I-xcb (downward triangles). a Profiles of cell growth (filled symbols) and xylose consumption (open symbols) under aerobic conditions. The strains were cultured in CGXIIA medium containing 20 g L−1 xylose in 500-mL flasks at 30 °C and 220 rpm with an initial OD600 of 0.8, and were induced with 0.5 mM IPTG. b SDS–PAGE analysis of intracellular proteins extracted from I-eco, I-ppm, I-sco and I-xcb from cultures with an OD600 of 5. Proteins were separated on a 12% SDS-PAGE gel. c Profiles of xylose consumption (open symbols) under anaerobic conditions with an initial OD600 of 30. 30 g L−1 4MgCO3·Mg(OH)2·5H2O, 200 mM sodium bicarbonate and 1 mM IPTG were added into the CGXIIB medium