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Table 1 Intercellular metabolites that were significantly higher or lower in concentration at below-standard pH values

From: Clostridium thermocellum LL1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics

Metabolite [retention time (min); key m/z] pH 6.48 vs 6.98 pH 6.24 vs 6.98α pH 6.12 vs 6.98 pH 5.80 vs 6.98
Fold change p value Fold change p value Fold change p value Fold change p value
Glutamic acid 57.5 0.429 267* 0.003 319 0.422 471 0.334
10.34 256 156 358 373 N-metabolite 30.4 0.432 83.3* 0.025 28 0.411 57.7* 0.014
3-Phosphoglyceric acid 1.98 0.052 45.2 0.384 10.8* 0.027 46.5 0.374
11.93 288 198 172 390 M+ N-metabolite 2.4 0.140 33* 0.000 14.1 0.194 6.17 0.291
Valine 4.75 0.127 16.7 0.082 4 0.118 12.4* 0.003
Mannose 6-phosphate 1.47* 0.020 14.1 0.364 3.33* 0.008 6.58 0.131
Adenine 2.68 0.509 11 0.122 4.83 0.463 16.6* 0.011
9.76 174 276 186 248 N-metabolite 2.53 0.414 9.21* 0.038 10.4 0.414 17.7 0.251
Alanine 1.87 0.194 8.86 0.145 6.16 0.202 11.3* 0.010
Monostearin 1.55 0.437 8 0.075 1.65* 0.040 15.5* 0.007
Stearic acid 1.84 0.173 7.96 0.174 2.26* 0.023 27.1 0.086
Glucose 6-phosphate 1.62 0.244 7.83 0.253 8.4* 0.004 9.3 0.128
10.81 218 202 320 100 N-metabolite 2.9 0.061 7.5 0.076 1.81 0.354 7.64* 0.024
13.30 299 315 357 328 211 P-metabolite 1.35 0.081 7.36* 0.017 7.52 0.120 29.6 0.088
15.23 218 203 244 N-metabolite 1.98 0.414 7.09* 0.017 2.58* 0.017 14.4 0.062
Lysine 1.74 0.496 6.67* 0.007 9.3 0.393 20.1 0.084
8.92 259 274 184 2.24 0.032 5.08 0.303 2.33* 0.009 8.27 0.141
15.2 347 glycoside 3.25 0.213 4.71 0.391 0.352** 0.006 1.18 0.174
6.96 245 260 102 organic acid 1.8 0.638 4.54 0.057 2.21 0.532 4.37* 0.013
Threonine 1.4 0.155 4.46 0.051 2.33* 0.038 1.52 0.100
Palmitic acid 2.03 0.139 4.17 0.305 2.54* 0.027 11.8* 0.001
Azelaic acid 1.15 0.573 3.33 0.368 1.55* 0.024 4.64 0.175
Isostearic acid 1.08 0.516 3.32 0.237 1.15 0.242 7.18* 0.015
10.89 218 191 100 362 2.5* 0.012 3.23 0.130 0.87 0.501 2.75* 0.028
11.33 299 211 328 415 175 P-metabolite 0.679 0.231 3.1* 0.026 3.09* 0.004 0.885 0.782
Glycine 1.53 0.391 2.67* 0.046 1.52 0.256 2.56 0.226
Isopalmitic acid 1.43 0.144 2.53 0.379 1.55 0.073 6.14* 0.033
Thymine 1.05 0.921 2.42 0.291 1.82 0.196 2.73* 0.036
Phosphate 1.28 0.451 2.21 0.158 1.73 0.125 3.02* 0.013
Citramalic acid 0.864 0.645 2.03 0.092 1.92* 0.041 2.9* 0.008
7.50 216 231 188 172 N-metabolite 1.73 0.524 2.01* 0.031 1.4 0.594 2.46 0.135
Glycerol 0.664 0.007 1.77 0.284 1.43* 0.037 2.86 0.241
Isoheptadecanoic acid 1.11 0.689 1.65 0.537 1.2 0.472 2.94* 0.020
Glucose 1.04 0.791 1.29 0.697 1.38* 0.021 2.79 0.215
Monopalmitin 0.879 0.774 1.2 0.710 1.6 0.107 3.87* 0.025
5′-Adenosine monophosphate 0.29** 0.009 0.644 0.589 2.72 0.364 0.531 0.349
2-Ethylbutylamine 0.591 0.072 0.57 0.058 0.4** 0.038 0.715 0.278
7.23 117 74 0.726 0.642 0.321** 0.015 0.835 0.708 0.489 0.071
15.90 292 uronic acid conjugate 0.268** 0.028 0.0691** 0.022 1.06 0.808 1.15 0.907
  1. αTable is ordered by descending fold change of intercellular metabolites at pH 6.24 compared to pH 6.98
  2. * Significantly higher at α = 0.05; ** significantly lower at α = 0.05