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Table 1 Intercellular metabolites that were significantly higher or lower in concentration at below-standard pH values

From: Clostridium thermocellum LL1210 pH homeostasis mechanisms informed by transcriptomics and metabolomics

Metabolite [retention time (min); key m/z]

pH 6.48 vs 6.98

pH 6.24 vs 6.98α

pH 6.12 vs 6.98

pH 5.80 vs 6.98

Fold change

p value

Fold change

p value

Fold change

p value

Fold change

p value

Glutamic acid

57.5

0.429

267*

0.003

319

0.422

471

0.334

10.34 256 156 358 373 N-metabolite

30.4

0.432

83.3*

0.025

28

0.411

57.7*

0.014

3-Phosphoglyceric acid

1.98

0.052

45.2

0.384

10.8*

0.027

46.5

0.374

11.93 288 198 172 390 M+ N-metabolite

2.4

0.140

33*

0.000

14.1

0.194

6.17

0.291

Valine

4.75

0.127

16.7

0.082

4

0.118

12.4*

0.003

Mannose 6-phosphate

1.47*

0.020

14.1

0.364

3.33*

0.008

6.58

0.131

Adenine

2.68

0.509

11

0.122

4.83

0.463

16.6*

0.011

9.76 174 276 186 248 N-metabolite

2.53

0.414

9.21*

0.038

10.4

0.414

17.7

0.251

Alanine

1.87

0.194

8.86

0.145

6.16

0.202

11.3*

0.010

Monostearin

1.55

0.437

8

0.075

1.65*

0.040

15.5*

0.007

Stearic acid

1.84

0.173

7.96

0.174

2.26*

0.023

27.1

0.086

Glucose 6-phosphate

1.62

0.244

7.83

0.253

8.4*

0.004

9.3

0.128

10.81 218 202 320 100 N-metabolite

2.9

0.061

7.5

0.076

1.81

0.354

7.64*

0.024

13.30 299 315 357 328 211 P-metabolite

1.35

0.081

7.36*

0.017

7.52

0.120

29.6

0.088

15.23 218 203 244 N-metabolite

1.98

0.414

7.09*

0.017

2.58*

0.017

14.4

0.062

Lysine

1.74

0.496

6.67*

0.007

9.3

0.393

20.1

0.084

8.92 259 274 184

2.24

0.032

5.08

0.303

2.33*

0.009

8.27

0.141

15.2 347 glycoside

3.25

0.213

4.71

0.391

0.352**

0.006

1.18

0.174

6.96 245 260 102 organic acid

1.8

0.638

4.54

0.057

2.21

0.532

4.37*

0.013

Threonine

1.4

0.155

4.46

0.051

2.33*

0.038

1.52

0.100

Palmitic acid

2.03

0.139

4.17

0.305

2.54*

0.027

11.8*

0.001

Azelaic acid

1.15

0.573

3.33

0.368

1.55*

0.024

4.64

0.175

Isostearic acid

1.08

0.516

3.32

0.237

1.15

0.242

7.18*

0.015

10.89 218 191 100 362

2.5*

0.012

3.23

0.130

0.87

0.501

2.75*

0.028

11.33 299 211 328 415 175 P-metabolite

0.679

0.231

3.1*

0.026

3.09*

0.004

0.885

0.782

Glycine

1.53

0.391

2.67*

0.046

1.52

0.256

2.56

0.226

Isopalmitic acid

1.43

0.144

2.53

0.379

1.55

0.073

6.14*

0.033

Thymine

1.05

0.921

2.42

0.291

1.82

0.196

2.73*

0.036

Phosphate

1.28

0.451

2.21

0.158

1.73

0.125

3.02*

0.013

Citramalic acid

0.864

0.645

2.03

0.092

1.92*

0.041

2.9*

0.008

7.50 216 231 188 172 N-metabolite

1.73

0.524

2.01*

0.031

1.4

0.594

2.46

0.135

Glycerol

0.664

0.007

1.77

0.284

1.43*

0.037

2.86

0.241

Isoheptadecanoic acid

1.11

0.689

1.65

0.537

1.2

0.472

2.94*

0.020

Glucose

1.04

0.791

1.29

0.697

1.38*

0.021

2.79

0.215

Monopalmitin

0.879

0.774

1.2

0.710

1.6

0.107

3.87*

0.025

5′-Adenosine monophosphate

0.29**

0.009

0.644

0.589

2.72

0.364

0.531

0.349

2-Ethylbutylamine

0.591

0.072

0.57

0.058

0.4**

0.038

0.715

0.278

7.23 117 74

0.726

0.642

0.321**

0.015

0.835

0.708

0.489

0.071

15.90 292 uronic acid conjugate

0.268**

0.028

0.0691**

0.022

1.06

0.808

1.15

0.907

  1. αTable is ordered by descending fold change of intercellular metabolites at pH 6.24 compared to pH 6.98
  2. * Significantly higher at α = 0.05; ** significantly lower at α = 0.05
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