Fig. 4

Effects of genome editing on 5′-UTR of mtlA gene for its reduced expression on carbon source consumption and metabolite productions. When three strains, KMK-01 (wild type, black bar), KMK-23 (dashed bar), and KIK-23M (genome-edited strain, gray bar), were cultured in the medium containing 40 g L⁻¹ glycerol and 20 g L⁻¹ mannitol, mannitol and glycerol consumption, 1,3-PDO production, microbial growth (OD₆₀₀), and 1,3-PDO production per unit mannitol consumption for 24 h were compared