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Table 4 Comparison of OD600, glycerol consumption, 1,3-PDO production and yield in KMK-12 and its mutants after 24 h of flask culturea

From: High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae

dhaD

 

Δ

 

Δ

glpK

  

Δ

Δ

Strain name

KMK-12

KMK-21

KMK-22

KMK-23

OD600

3.53

3.26

3.32

3.85

Consumed glycerol (g L−1)

10.49

7.49

13.56

7.59

Consumed glucose (g L−1)

7.80

9.24

7.15

11.97

Acetate production (g L−1)

0

2.25

1.47

1.78

Succinate production (g L−1)

0.23

0.14

0.20

0.25

1,3-PDO production (g L−1)

4.87

4.79

6.09

5.27

1,3-PDO productivity (g L−1 h−1)

0.20

0.20

0.25

0.22

1,3-PDO yield (mol mol−1)b

0.56

0.77

0.54

0.84

  1. a Experiments were repeated three times independently
  2. b Yield is calculated as dividing produced metabolite by consumed glycerol