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Table 4 Comparison of OD600, glycerol consumption, 1,3-PDO production and yield in KMK-12 and its mutants after 24 h of flask culturea

From: High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae

dhaD   Δ   Δ
glpK    Δ Δ
Strain name KMK-12 KMK-21 KMK-22 KMK-23
OD600 3.53 3.26 3.32 3.85
Consumed glycerol (g L−1) 10.49 7.49 13.56 7.59
Consumed glucose (g L−1) 7.80 9.24 7.15 11.97
Acetate production (g L−1) 0 2.25 1.47 1.78
Succinate production (g L−1) 0.23 0.14 0.20 0.25
1,3-PDO production (g L−1) 4.87 4.79 6.09 5.27
1,3-PDO productivity (g L−1 h−1) 0.20 0.20 0.25 0.22
1,3-PDO yield (mol mol−1)b 0.56 0.77 0.54 0.84
  1. a Experiments were repeated three times independently
  2. b Yield is calculated as dividing produced metabolite by consumed glycerol